We narrowed to 19,233 results for: INO

1. iRFP-FRB1C-Cb5

   Plasmid

   #162441

   Purpose

   for use in CIT (chemically induced trimerization) system

   Depositor

   Takanari Inoue

   Article

   Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

   Insert

   iRFP-FRB1C-Cb5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-21a(+)-DR RecR

   Plasmid

   #12617

   Depositor

   SeWon Suh

   Article

   Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

   Insert

   Deinococcus radiodurans RecR (recR Deinococcus radiodurans)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lyn-YFP-FKBP1N

   Plasmid

   #162437

   Purpose

   for use in CIT (chemically induced trimerization) system

   Depositor

   Takanari Inoue

   Article

   Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

   Insert

   Lyn-YFP-FKBP1N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BS.YxiN.Twin1.a1-479.wt

   Plasmid

   #13527

   Depositor

   David McKay

   Article

   Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

   Insert

   B. subtilis DEAD-box helicase YxiN
   

   Use

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T7-WbpB-His6

   Plasmid

   #89711

   Purpose

   Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   WbpB (wbpB Pseudomonas aeruginosa)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T7-WbpE-His6

   Plasmid

   #89712

   Purpose

   Expresses WbpE from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   WbpE (wbpE Pseudomonas aeruginosa)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. T7-WbpD-His6

   Plasmid

   #89713

   Purpose

   Expresses WbpD from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   WbpD (wbpD Pseudomonas aeruginosa)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_NKX2-1

   Plasmid

   #221472

   Purpose

   Gateway cloning of NKX2-1 short isoform overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGAD424-AP-4 σ4

   Plasmid

   #198328

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-4 σ4
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lyn-YFP-FRB3C

    Plasmid

    #162427

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    Lyn-YFP-FRB3C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FRB3N-CFP-CAAX

    Plasmid

    #162430

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    FRB3N-CFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YFP-FRB1N-Cb5

    Plasmid

    #162435

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    YFP-FRB1N-Cb5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FRB2N-CFP-CAAX

    Plasmid

    #162429

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    FRBN-CFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lyn-YFP-FRB2C

    Plasmid

    #162426

    Purpose

    for use in CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    Lyn-YFP-FRB2C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTomatoSlicer

    Plasmid

    #221085

    Purpose

    Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VPS13D^EGFP

    Plasmid

    #174109

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    With an internal EGFP tag in aminoacid position 1…

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET29b-IPP1-His

    Plasmid

    #124137

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Inorganic pyrophosphatase (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-SUPREM

    Plasmid

    #205023

    Purpose

    Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    SUPer Rna Ecogii Methyltransferase
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHA2

    Plasmid

    #85710

    Purpose

    This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

    Depositor

    Ulrich Koszinowski, Martin Messerle

    Article

    Adler et al J Virol. 2000 Aug;74(15):6964-74.

    Type

    Empty backbone

    Use

    Cre/Lox; Herpesvirus cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 5HT6-mCherry-G-GECO1.0

    Plasmid

    #47500

    Purpose

    Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    G-GECO1.0
    

    Use

    Tags

    5HT6 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RUFY1-GFP

    Plasmid

    #197394

    Purpose

    Expresses RUFY1 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Keren-Kaplan et al Nat Commun. 2022 Mar 21;13(1):1506. doi:

    Insert

    RUFY1 (RUFY1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

    Plasmid

    #71878

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC-delta (Otc Rat)
    

    Use

    Tags

    Expression

    Mutation

    Deleted amino acid 30-114 from full length OTC

    Promoter

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lyn11-targeted FRB (LDR)

    Plasmid

    #20147

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-linker-FRB (LDR) (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. flag-RapR-PTP1B

    Plasmid

    #188656

    Purpose

    Rapamycin regulated PTP1B with flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    PTP1B
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Tom20-mCh-FKBP

    Plasmid

    #162436

    Purpose

    for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    Tom20-mCh-FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b-pchG WT

    Plasmid

    #87107

    Purpose

    expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

    Insert

    pchG WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RP4-6

    Plasmid

    #80385

    Purpose

    Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Spectinomycin/Streptomycin
    

    Use

    Helper bhr plasmid for mobilizing other plasmids …

    Tags

    none

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

32. VPS13D^mScarlet

    Plasmid

    #174110

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    With an internal mScarlet tag in aminoacid positi…

    Promoter

    CMV

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Let-7b-GFP

    Plasmid

    #25404

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    Mirlet7b (Mirlet7b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

34. RSRF-Luc-2wt

    Plasmid

    #31818

    Depositor

    Astar Winoto

    Article

    Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

    Insert

    MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MEF2-dependent (-307 to -242 of Nur77/NR4A1)

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-HA-Uch37

    Plasmid

    #19415

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 (UCHL5 Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCI-myc-USP10

    Plasmid

    #184034

    Purpose

    Expresses myc-USP10 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

    Insert

    USP10 (USP10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMVf

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKm-HCBE

    Plasmid

    #170333

    Purpose

    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized CBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKm-HGBE

    Plasmid

    #170334

    Purpose

    a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized GBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge