We narrowed to 4,241 results for: Pet2

1. pET28a(His10-PD-1cyto)

   Plasmid

   #177896

   Purpose

   E coli expression of His10 tag fused to human PD-1 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   PD-1 (aa 194-288 only) (PDCD1 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-ClbPpep-CHis

   Plasmid

   #48243

   Purpose

   First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP-pep
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   First 375 amino acids of the peptidase, which con…

   Promoter

   T7

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-Y986H

   Plasmid

   #174801

   Purpose

   Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986H (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1-Y986S

   Plasmid

   #174802

   Purpose

   Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986S (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_6xHis-PARP1-G972R

   Plasmid

   #174800

   Purpose

   Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G972R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G972R, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5787 pET28aSX104B/ pES42

   Plasmid

   #1229

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-pro-sumo3

   Plasmid

   #25103

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO3 (SUMO3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Serine 2 to Alanine

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET22b-T5-FB-E25TAG-F6C

   Plasmid

   #176546

   Purpose

   Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change phenylalanine 6 to cysteine; change glutam…

   Promoter

   T5

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

12. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Brg1 Del3 pET28a

    Plasmid

    #122117

    Purpose

    Expression Brg1 amino acids 836-1446 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Brg1 Del1 pET28a

    Plasmid

    #122115

    Purpose

    Expression Brg1 amino acids 1-381 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-SUMO-KaiB

    Plasmid

    #68050

    Purpose

    Strain: AM5116, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiB
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-DDX43-K292A

    Plasmid

    #134575

    Purpose

    Express human DDX43 mutant (K292A, ATPase dead) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-K292A (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (K292A)

    Promoter

    T7

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-SUMO-KaiA

    Plasmid

    #68049

    Purpose

    Strain: AM5115, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiA
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-PKM2-G415R

    Plasmid

    #122693

    Purpose

    Express His-tagged PKM2 (G415R) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    G415R

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eGFP-Silicatein-pET28

    Plasmid

    #198012

    Purpose

    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

    Depositor

    Bryan Berger

    Article

    Vigil et al Materials Advances 2, 2023

    Insert

    eGFP-silicatein
    

    Use

    Tags

    hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-p53 (1-73) (72P)

    Plasmid

    #62083

    Purpose

    Expression of human p53 (1-73) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72P

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pET28b(+)-SPN+IFS

    Plasmid

    #83372

    Purpose

    Expresses SPN and IFS complex in E. coli cell

    Depositor

    SeWon Suh

    Article

    Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

    Inserts

    SPN
    IFS
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-Beclin1 (1-242)

    Plasmid

    #24401

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-242 only.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21a- hPTP1e-PDZ2

    Plasmid

    #20377

    Depositor

    Andrew Lee

    Article

    Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

    Insert

    hPTP1e PDZ2 (PTPN13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET20b-delta133HtrA2 A141S

    Plasmid

    #16157

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A141S

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET24a-LaM4-1xTS

    Plasmid

    #162778

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21a-mAIM2-deltaPYD

    Plasmid

    #51535

    Purpose

    Express His6-tagged HIN domain of mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28 Set9 mutant

    Plasmid

    #24083

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    H297A

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

34. TrmD_HaeIn/pET28b(+) N,C-tag

    Plasmid

    #12665

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21-pelB-LaG-24

    Plasmid

    #172750

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-24
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28-p53 (Mouse 1-87)

    Plasmid

    #62061

    Purpose

    Expression of Mouse p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pET20b- hTPI Lys13Arg

    Plasmid

    #50726

    Purpose

    overexpression of the human TPI1 Lys13Arg allele in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    T7

    Available since

    Feb. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge