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We narrowed to 1,446 results for: pSC

Showing: 561 - 580 of 1446 results

  3. pTUM4

    Plasmid
    #230905
    Purpose
    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli
    Depositor
    Arne Skerra
    Article
    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-
    Inserts
    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    230905-plasmid-map-sequence-id-460506

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  4. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Article
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Insert
    CACNA1D (Cacna1d Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49332-plasmid-map-sequence-id-215242

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  5. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid
    #122056
    Purpose
    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P
    Depositor
    Stephen Tapscott
    Article
    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do
    Insert
    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    MyoD chimeric protein substituted with the NeuroD…
    Promoter
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122056-plasmid-map-sequence-id-235399

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  10. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

    Plasmid
    #217893
    Purpose
    Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217893-plasmid-map-sequence-id-432152

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  15. AAVS1-Hb9-CD14

    Plasmid
    #204344
    Purpose
    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    human CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hb9 (Mnx1) 9kb promoter fragment
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    204344-plasmid-map-sequence-id-407654

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  16. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124230-plasmid-map-sequence-id-240604

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  18. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

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  19. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

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  20. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter and IRES
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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Showing: 561 - 580 of 1446 results