We narrowed to 5,323 results for: pcDNA3.1

1. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-MS2-HA-HsSmg7_T

   Plasmid

   #147556

   Purpose

   Mammalian Expression of HsSmg7

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg7 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutation G216A, A1521G and one non sil…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1/cyto-iAspSnFR

   Plasmid

   #201394

   Purpose

   CMV overexpression of cyto-iAspSnFR in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

   Insert

   cyto-iAspSnFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

   Promoter

   CMV

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY016 (pcDNA3.1-hLbCpf1)

   Plasmid

   #69988

   Purpose

   Expresses humanized LbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-N-Myc_WT USP7

   Plasmid

   #131242

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-ccdB-Nanoluc

   Plasmid

   #87067

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+)-C-6His-mEsrra

   Plasmid

   #173152

   Purpose

   mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Article

   Tripathi et al bioRxiv 2021.11.16.468891

   Insert

   ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-Peredox-mCherry

   Plasmid

   #32383

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   Peredox-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pF155 pcDNA3.1(+)SOD1G93A

   Plasmid

   #26401

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G93A

   Promoter

   Available Since

   Nov. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

    Plasmid

    #159128

    Purpose

    expresses KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-VEGFR2-V5/HIS

    Plasmid

    #205617

    Purpose

    Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR2 receptor tyrosine kinase (KDR Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-INSR-V5/HIS

    Plasmid

    #201980

    Purpose

    expression of human INSR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSR receptor tyrosine kinase (INSR Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-GGamma9-GFP2

    Plasmid

    #140991

    Purpose

    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma9-GFP2 (GNGT2 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-20nm-EBFP2

    Plasmid

    #231185

    Purpose

    Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    20nm-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid

    #148120

    Purpose

    Mammalian Expression of HshnRNPH1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPH1 (HNRNPH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

    Plasmid

    #159129

    Purpose

    expresses NRF2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. alpha-0 CFP in pcDNA3.1

    Plasmid

    #36396

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein alpha 0 (Gnao1 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted into the KpnI-SpeI sites engineered …

    Promoter

    cmv

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. opto-RhoGEF-mCherry_pcDNA3.1

    Plasmid

    #164473

    Purpose

    Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoGEF
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Flag-NPM1c

    Plasmid

    #131813

    Purpose

    Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    This is the cytoplasmic mutant causing AML, where…

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_GEM2-Halo-FRB

    Plasmid

    #197056

    Purpose

    Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge