We narrowed to 15,119 results for: RAP

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VAMP4-SEP

Plasmid

#174406

Purpose

Reporter for VAMP4 exocytosis in mammalian cells

Depositor

David Perrais

Article

Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

Insert

vesicle associated membrane protein 4 (VAMP4 Human)

Tags

superecliptic phulorin (mutated GFP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

Plasmid

#128268

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(III)-GBPen

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUTKan-3xHA-GFP-TOM5

Plasmid

#130674

Purpose

Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

Depositor

Andreas Weber

Article

Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

Insert

Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)

Tags

3xHA and sGFP

Expression

Plant

Promoter

Ubiquitin10

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-11

Plasmid

#72349

Purpose

Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRDB_182

Plasmid

#216103

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Lyn-FKBP-FKBP-CFP (LF2C)

Plasmid

#20149

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

Lyn-FKBP-FKBP-CFP (FKBP1A Human)

Expression

Mammalian

Mutation

See depositor comments below.

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMK294 (mAID-mCherry2-Bsr)

Plasmid

#72832

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Bsr

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-Map4

Plasmid

#105571

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

MAP4 (Map4 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-NeoR-CAG-EGFPnls-WPRE

Plasmid

#191692

Purpose

For knocking pCAG-EGFPnls into AAVS1 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

AAVS1 homology arms and pCAG-EGFPnls

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-mem-seTurboID-IRES-tTA

Plasmid

#237889

Purpose

Amplifier AAV vector of Dual-AAV sparse labeling system encoding membrane-tethered seTurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

mem-seTurboID

Use

AAV

Tags

FLAG tag and GAP43 palmitoylation signal

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pC081: pAAV.CMV.mCherry

Plasmid

#205744

Purpose

AAV vector for expressing CMV-driven mCherry reporter gene.

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

Plasmid

#199354

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDawn-Ag43

Plasmid

#107742

Purpose

Expresses Ag43 under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P199L

Plasmid

#177949

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P199L) (N )

Expression

Mammalian

Mutation

P199L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-532)

Plasmid

#37314

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-532

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV tdTomato L5-L2

Plasmid

#62152

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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AALN-BE4max

Plasmid

#138161

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(AALN)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Ii-Str_TNF-SBP-EGFP

Plasmid

#65280

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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