164,884 results

1. pGWB501

   Plasmid

   #74843

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LifeAct-mNeonGreen

   Plasmid

   #98877

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   LifeAct
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPneogRNA241510+MT

   Plasmid

   #84292

   Purpose

   Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
   

   Use

   CRISPR; Leishmania donovani

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBTet-SNAP-STIL

   Plasmid

   #136795

   Purpose

   Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-STIL (STIL Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-LMNA-gRNA1

   Plasmid

   #122507

   Purpose

   expresses WT spCas9 and a chimeric gRNA targeting human LMNA

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   LMNA-gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat (AAV1)

   Viral Prep

   #169128-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-DIO-EF1α-mKate2-PDE4D3-Cat (#169128). In addition to the viral particles, you will also receive purified pAAV-DIO-EF1α-mKate2-PDE4D3-Cat plasmid DNA. EF1a-driven, Cre-dependent expression of truncated PDE4D3 and mKate2. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Promoter

   EF1a

   Tags

   None

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

7. PX552-mScn2a-3xV5-EF1-smFP-flag

   Plasmid

   #182561

   Purpose

   For mouse Nav1.2 knockin with 3xV5 at the C-terminal

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Inserts

   mouse Scn2a gRNA and 3xV5 donor DNA
   smFP-flag
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1 and U6

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHybr_r2a>mA-srt-his

   Plasmid

   #124806

   Purpose

   HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.

   Depositor

   Martijn Verdoes, Ferenc Scheeren

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgA
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB179-TurboPETase

   Plasmid

   #218693

   Purpose

   Encodes codon optimized TurboPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TurboPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2_AAVS1_sgRNA_2

    Plasmid

    #155088

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNG6

    Plasmid

    #30990

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NG6
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHEE901-A3A

    Plasmid

    #229044

    Purpose

    nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRECMYC-Ebu1

    Plasmid

    #240713

    Purpose

    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

    Depositor

    Seth Shipman

    Insert

    Ebu1 RT, Eco1 ncRNA, MspRecT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid

    #236097

    Purpose

    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLD-puro-Cc-SARS-CoV-2_NSP16-VA

    Plasmid

    #191215

    Purpose

    Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

    Depositor

    Mohan Babu

    Insert

    Non structural protein 16 (ORF1ab SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTR01F/hFGFR2b-V5

    Plasmid

    #236014

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2b protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2b (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJAK184.tetR.PT5-3.dCas9

    Plasmid

    #232482

    Purpose

    optimized ahTet inducible dCas9 for insertion into C. difficile genome

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Insert

    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-PT5-3

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC.15A.sgRNA.TA

    Plasmid

    #232483

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Inserts

    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 constitutive promoter and native promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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