We narrowed to 32,648 results for: Eng

1. pAAV-hSyn-Cepheid1s-ST-WPRE

   Plasmid

   #214968

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

   Depositor

   Peng Zou

   Article

   Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

   Insert

   Cepheid1s-ST
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO-HRASG12V

   Plasmid

   #216509

   Purpose

   Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   HRAS (HRAS Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12V

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221 UBR5

   Plasmid

   #81062

   Purpose

   Gateway entry vector encoding full length UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
   

   Use

   Cloning

   Mutation

   K503R

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-sgRNA-lib 2.0

   Plasmid

   #89638

   Purpose

   sgRNA expression in mammalian cells after Gateway cloning

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HH02-LP Clone 2 heavy chain

   Plasmid

   #192175

   Purpose

   Clone 2 heavy chain (HH02)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 heavy chain (HH02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HK02-LP Clone 2 light chain

   Plasmid

   #192179

   Purpose

   Clone 2 light chain (HK02)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 light chain (HK02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

   Plasmid

   #89888

   Purpose

   Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-KRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Myc CDC4 WT*

   Plasmid

   #16652

   Depositor

   Bert Vogelstein

   Article

   Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

   Insert

   CDC4 (FBXW7 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   *Note that this insert is only WT relative to the…

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Tags

    Cerulean

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT3 Unc5B-FLAG

    Plasmid

    #72195

    Purpose

    Expresses full-length Unc5b with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b (Unc5b Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTT3 Unc5A-FLAG

    Plasmid

    #72194

    Purpose

    Expresses full-length Unc5a with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a (Unc5a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. sg_Brd9_i5-ipUSEPR-TR657

    Plasmid

    #228922

    Purpose

    Knockdown of Brd9 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Brd9_i5 (Brd9 Mouse)
    

    Use

    Lentiviral

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. sg_Brd9_i2-ipUSEPR-TR657

    Plasmid

    #228921

    Purpose

    Knockdown of Brd9 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Brd9_i2 (Brd9 Mouse)
    

    Use

    Lentiviral

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX459-TP53-exon4

    Plasmid

    #217455

    Purpose

    For TP53 knockout targeting exon 4 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTT3 Unc5D-FLAG

    Plasmid

    #72197

    Purpose

    Expresses full-length Unc5d with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d (Unc5d Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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