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7M8

Plasmid

#64839

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

Insert

7M8 cap

Use

AAV

Mutation

7mer insertion in the AAV2 cap

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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AB-GFP

Plasmid

#72203

Purpose

screen AB42 variants for aggregation

Depositor

Michael Hecht

Article

Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

Insert

AB42 (APP Human)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-rab11 WT

Plasmid

#12674

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD3 Flag

Plasmid

#74895

Purpose

Express COMMD3 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD3 (COMMD3 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MCS-GFP-SV-puro

Plasmid

#73582

Purpose

Third generation lentiviral vector expressing GFP and puromycin

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

GFP tag

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (1-1304)

Plasmid

#74244

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Expression

Mammalian

Mutation

Contains ASXL1 amino acids 1-1304

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-Rab4a-7

Plasmid

#56440

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab4a (RAB4A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/FGFR2myc

Plasmid

#33248

Depositor

Claudio Basilico, Alka Mansukhani

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Tags

His and Myc

Expression

Mammalian

Mutation

KpnI site introduced at stop codon of FGFR2 to be…

Promoter

CMV

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
LYN11-FRB-CFP

Plasmid

#38003

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

LYN (LYN Human)
MTOR (MTOR )

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

Plasmid

#84561

Purpose

pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

Depositor

Robert Singer

Article

Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

Insert

FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops

Use

Lentiviral

Tags

Flag, Suntag, oxBFP, AID (auxin-inducible degron)

Promoter

UbC

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1

Plasmid

#13798

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Article

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Article

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Tags

His and roGFP2

Expression

Bacterial

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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