We narrowed to 13,844 results for: cas9 genes

1. pET28b-DiCas7-11-His

   Plasmid

   #234482

   Purpose

   Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEN35-CDKN2A-Ex2-R

   Plasmid

   #110737

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEN35-CDKN2A-Ex2-L

   Plasmid

   #110736

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWZ194

   Plasmid

   #163640

   Purpose

   rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)
   

   Use

   CRISPR

   Tags

   2xmKate2 and GFP

   Expression

   Worm

   Mutation

   Promoter

   rps-27

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pINSPECT:IL2_SigP:NLuc

   Plasmid

   #197266

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   Interleukin-2 homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pINSPECT:NEAT1-v2_SigP:NLuc

   Plasmid

   #197268

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-v2 homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAWK61

   Plasmid

   #163642

   Purpose

   rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Nematode, Human)
   

   Use

   CRISPR

   Tags

   2xmKate2 and GFP

   Expression

   Worm

   Mutation

   Promoter

   rps-27

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pINSPECT:NEAT1-total_SigP:NLuc

   Plasmid

   #197267

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-total homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-Blast-sgMARK3

   Plasmid

   #138706

   Purpose

   Expresses a human MARK3-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgMARK3 human (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Oxford Drosophila melanogaster Genome-wide CRISPR Library

    Pooled Library

    #64750

    Purpose

    A genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).

    Depositor

    Ji-Long Liu

    Article

    Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-

    Expression

    Insect

    Species

    Drosophila melanogaster, Streptococcus pyogenes, and Synthetic

    Use

    CRISPR

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pXPR_118

    Plasmid

    #113667

    Purpose

    Lentiviral vector expressing dCas9

    Depositor

    John Doench

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    catalytically inactive

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PLJR965_Rv3297

    Plasmid

    #241235

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv3297) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv3297 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PLJR965_Rv2976c

    Plasmid

    #241234

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv2976c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv2976c sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PLJR965_Rv2464c

    Plasmid

    #241232

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv2464c sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PLJR965_Rv1638

    Plasmid

    #241231

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1638) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1638 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PLJR965_Rv1259

    Plasmid

    #241228

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1259) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1259 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PLJR965_Rv1020

    Plasmid

    #241227

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1020) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1020 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PLJR965_Rv0949

    Plasmid

    #241226

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv0949) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv0949 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLJR965_Rv1633

    Plasmid

    #241230

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1633) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1633 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PLJR965_Rv3589

    Plasmid

    #241236

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv3589) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv3589 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PLJR965_Rv2924c

    Plasmid

    #241233

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv2924c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv2924c sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PLJR965_Rv1420

    Plasmid

    #241229

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1420) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1420 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

    Plasmid

    #217306

    Purpose

    sgRNA mcherry + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid

    #136271

    Purpose

    Expresses PE2 and Tetra-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2 and pegRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid

    #136272

    Purpose

    Expresses PE2 and ST2-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2, pegRNA scaffold
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

    Plasmid

    #217305

    Purpose

    sgRNA BFP + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LZ3 dFE

    Plasmid

    #190144

    Purpose

    Expresses LZ3Cas9-T4pol (LZ3 dFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    LZ3Cas9-T4pol (LZ3 dFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LZ3 iFE

    Plasmid

    #190143

    Purpose

    Expresses LZ3Cas9-POLB (LZ3 iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    LZ3Cas9-POLB (LZ3_iFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGH044_mCherry

    Plasmid

    #85412

    Purpose

    lentiviral expression vector for mCherry

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJ1996_v2

    Plasmid

    #140664

    Purpose

    dCas9 & Csy4

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    dCas9 & Csy4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCRISPRainbow-DONOR1

    Plasmid

    #75398

    Purpose

    CRISPRainbow-DONOR1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    Cm(R)-CcdB
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mutation

    Promoter

    Bacteria

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGH045_EGFP

    Plasmid

    #85411

    Purpose

    lentiviral expression vector for GFP

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGH220_wtGFP

    Plasmid

    #85410

    Purpose

    lentiviral expression vector for wtGFP

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    wtGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBH501

    Plasmid

    #215780

    Purpose

    NBU1 backbone with constitutive dCas9

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pV1081

    Plasmid

    #111425

    Purpose

    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgADE-NT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFD152

    Plasmid

    #125546

    Purpose

    derivative of pFD116 with an optimized RBS for E. coli

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Insert

    optimized RBS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    anhydrotetracycline Ptet promoter

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOP576

    Plasmid

    #85943

    Purpose

    Temperature sensitive plasmid based on pDK46. Expresses dCas9 from proD. Serves as a control for pOP587 - pOP594

    Depositor

    Timothy Lu

    Article

    Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAK005

    Plasmid

    #233642

    Purpose

    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    eSpCas9(1.1) with c-terminal VitelloTagB
    

    Use

    Tags

    6-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOSIP-KL-mcherry

    Plasmid

    #116947

    Purpose

    Integrating mCherry into lambda attB in E. coli chromosome.

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    mcherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge