We narrowed to 579 results for: SPY
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Plasmid#243789PurposeExpresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A-SpyTag
Plasmid#243769PurposeExpresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S-SpyTag
Plasmid#243770PurposeExpresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-T-SpyTag
Plasmid#243771PurposeExpresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A OR S-SpyTag
Plasmid#243772PurposeExpresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A OR T-SpyTag
Plasmid#243773PurposeExpresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S OR T-SpyTag
Plasmid#243774PurposeExpresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tagExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A AND S-SpyTag
Plasmid#243775PurposeExpresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A AND T-SpyTag
Plasmid#243776PurposeExpresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S AND T-SpyTag
Plasmid#243777PurposeExpresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern- A AND (S OR T)-SpyTag
Plasmid#243778PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND (sortase eSrtA(4S9) OR TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern- S AND (A OR T)-SpyTag
Plasmid#243779PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND (sortase eSrtA(2A9) OR TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern- T AND (A OR S)-SpyTag
Plasmid#243780PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to TEV AND (sortase eSrtA(2A9) OR sortase eSrtA(4S9)) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
Tags6x His tag and SsrAExpressionBacterialAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TwinStrepSUMO-Nb-SpyCatcher
Plasmid#243752PurposeFor affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcherDepositorInsertNb-SpyCatcher
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_Z11 gpN:SpyTag
Plasmid#225196PurposeUsed for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-P-Domain
Plasmid#201193PurposeExpresses furin P-domain genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-P-Domain
TagsHisTag and SpyCatcher001ExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
TagsHis10, AP fragment of split APEX2 tagExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2796 (pSpy0C4)
Plasmid#220280Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombinationDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA-GFP
Plasmid#220189PurposesgRNA expression vector - pEM7 promoter with GFP in sgRNA siteDepositorInsertpEM7 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpEM7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD-GFP
Plasmid#220192PurposesgRNA expression vector - pBG28 promoter with GFP in sgRNA siteDepositorInsertpBG28 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG28Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Quartet-SpyTag
Plasmid#214726PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag fusionDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet
Plasmid#214727PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet_NoLinker
Plasmid#214731PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTagDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-DIIG
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
TagsHisTagExpressionBacterialPromoterT7Available SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
TagsHisTagExpressionBacterialPromoterT7Available SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
TagsHisTagExpressionBacterialPromoterT7Available SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B4.SPy1128
Plasmid#11531DepositorInsertphosphotransacetylase
TagsHisTag-MBP, TEV cleavableExpressionBacterialAvailable SinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
TagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcher-sfGFP
Plasmid#107420PurposeFor production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
TagsHis tag, StrepII, and superfolder GFPExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
ExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-HA2
Plasmid#165991PurposeSpyCatcher-fused influenza HA ministem with removable HisTagDepositorInsertSpyCatcher-HA2
TagsFoldon, HisTag, and SpyCatcherExpressionBacterialPromoterT7Available SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
His6-SpyCatcher-mi3
Plasmid#113043PurposeExpresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertHis6-SpyCatcher-mi3
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
TagsHis6 tag and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
TagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
ExpressionBacterialPromoterT7Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
TwinStrepSUMO-Ad5-SpyCatcher
Plasmid#243751PurposeFor affinity purification of A5 knob fused to SpyCatcherDepositorInsertAd5-SpyCatcher
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10
Plasmid#243740PurposePVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10)DepositorInsertpvc13-SpyTag_Δpvc10
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher-mi3
Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
TagsC-tagExpressionBacterialPromoterT7Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21 MBPx-SpyCatcher
Plasmid#72327PurposeBacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.DepositorInsertpET21 MBPx-SpyCatcher
TagsN-terminal His6 tagExpressionBacterialMutationMBP contains A312V, I317V mutations and 172, 173,…Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)
Plasmid#194217PurposeLentiviral transducition vector for a GAL4-responsive tagBFP reporter geneDepositorInsertUAS-ybTATA-tagBFP; PGK-mCitrine
UseLentiviralPromoterGAL4 UAS-ybTATA; PGKAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD86
Plasmid#223598PurposeLentiviral expression of human CD86 with SpyTag003 in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only