We narrowed to 281 results for: dcas9 vp64

1. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Promoter

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-Gal4-dCas9-VP64

   Plasmid

   #71128

   Purpose

   This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

   Depositor

   Ronald Davis

   Article

   Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

   Insert

   Gal4-dCas9-VP64
   

   Use

   Tags

   NLS n-terminal, N terminal Gal4 Activator domain,…

   Expression

   Yeast

   Mutation

   Promoter

   Tef1

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-dCas9(sa)-VP64-AAV

   Plasmid

   #213035

   Purpose

   dCas9-VP64 expressed under control of TRE

   Depositor

   Nadav Ahituv

   Article

   Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

   Insert

   dCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and NLS-VP64

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pb-CAGGS-dCas9-VP64-blasto

   Plasmid

   #218556

   Purpose

   Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance

   Depositor

   Alejandro Chavez

   Insert

   dCas9
   

   Use

   CRISPR; Piggybac transposition

   Tags

   2xSV40NLS-VP64

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Expression

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-LTR-dCas9-VP64-BFP

   Plasmid

   #46912

   Purpose

   Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   dCas9-VP64-BFP fusion
   Puromycin resistance
   

   Use

   CRISPR and Retroviral

   Tags

   3xNLS, BFP, and VP64 domain

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and PGK

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti Vp64-dCas9 T2A mTagBFP2

    Plasmid

    #171052

    Purpose

    Expression of Vp64-dCas9 activation system

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    Vp64-dCas9 T2A mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/hUBC

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hUbC-dCas9 VP64-T2A-GFP

    Plasmid

    #53192

    Purpose

    Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101102

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101107

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101115

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    LPAR1-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101113

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    CXCR4-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101111

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to AVPR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    AVPR2-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge