We narrowed to 8,863 results for: sgRNA

1. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133348

   Purpose

   expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA and sgRNA_cpaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSGKP_AcsgRNA

   Plasmid

   #203807

   Purpose

   pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcgRNA
   

   Use

   CRISPR

   Promoter

   J23119

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. psgRNA

   Plasmid

   #114005

   Purpose

   express sgRNA. ColE1, Kan

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   gRNA
   

   Promoter

   BBa_J23119

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6>sgRNA(F+E)

   Plasmid

   #59986

   Purpose

   Empty vector for sgRNA cloning (F+E modified backbone)

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   Ciinte.U6

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. psgRNAc

   Plasmid

   #114006

   Purpose

   express sgRNA, p15A, CRM

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   gRNA
   

   Promoter

   BBa_J23119

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_PHF19_sgRNA

   Plasmid

   #246404

   Purpose

   Cas9/sgRNA expression plasmid targeting PHF19

   Depositor

   Yicheng Long

   Article

   Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

   Insert

   PHF19 (PHF19 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6b:sgRNA#3

   Plasmid

   #64247

   Purpose

   expresses sgRNA under U6b promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. EC2_10_dCas9_VPR_HC_sgRNA

    Plasmid

    #163712

    Purpose

    Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA_expression_vector

    Plasmid

    #210212

    Purpose

    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

    Depositor

    Albert Jeltsch

    Article

    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATP1A1_G8_Dual_sgRNA

    Plasmid

    #178104

    Purpose

    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 sgRNA + user-specified sgRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. px458_2A_GFP_sgRNA_LTN1

    Plasmid

    #127125

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA LTN1 (LTN1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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