We narrowed to 3,055 results for: SRS
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Plasmid#31679DepositorInsertProtein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)
UseLentiviralTagsM2ExpressionMammalianMutationchanged Serine 452 to AlanineAvailable SinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-DEST
Plasmid#119745PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)DepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-AMPKalpha2 wt
Plasmid#31654DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
TagsHA (YDVPDYASL)ExpressionMammalianAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-GFP
Plasmid#82506PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.DepositorInserteGFP
UseSynthetic BiologyExpressionMammalianAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shControl
Plasmid#180386PurposeProducing AAV that encodes negative control shRNA with miR-E backboneDepositorInsertshControl
UseAAV and RNAiPromoterCBAAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.HA_mCherry-NLS
Plasmid#178209PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.HA and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
TagsHA tag and Histidine tagExpressionBacterialPromoterT7Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.NWS_mCherry-NLS
Plasmid#178282PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.NWS and Nuclear Localization SignalPromotereF1aAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVExpressionMammalianAvailable SinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.VSVg_mCherry-NLS
Plasmid#178214PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.VSVg and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS CsChrimson tdtomato_tr
Plasmid#111544PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertChrimson_tdtomato_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.V5_mCherry-NLS
Plasmid#178215PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.V5 and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CoChR-GFP
Plasmid#59070PurposeAAV-mediated expression of CoChR-GFP under the Syn promoterDepositorInsertCoChR-GFP
UseAAVTagsGFPExpressionMammalianPromoterSynAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pHSE::Cre-mRuby3
Plasmid#164136PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat inductionDepositorInsertHSE promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…ExpressionMammalianPromoterHSE synthetic promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralMutationD64V mutation within the integrase within PolAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS ChrimsonR mCherry_tr
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FCK-ChrimsonR-GFP
Plasmid#59049PurposeLentiviral expression of ChrimsonR under the CaMKII promoterDepositorInsertChrimsonR-GFP
UseLentiviralTagsGFPExpressionMammalianMutationK176RPromoterCaMKIIAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-CDC27 wt
Plasmid#31661DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)
Plasmid#171101PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.DepositorAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
TagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…Available SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP
Plasmid#164131PurposeExpresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)DepositorAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-Tq2CFP-OcsT
Plasmid#71268PurposeEntry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTq2CFP
UseGateway CloningTags4xGly linker and octaline synthase terminatorAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Cep290
Plasmid#27380DepositorInsertCep290 (Cep290 Mouse)
TagsmCherryExpressionMammalianMutationplease see depositor comments belowAvailable SinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mCep290
Plasmid#27379DepositorAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-GUS-OcsT
Plasmid#71267PurposeEntry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertBeta-glucuronidase
UseGateway CloningTags2xGly linker and octaline synthase terminatorAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
5XUAS IVS CsChrimson tdtomato_tr
Plasmid#111545PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertCsChrimson_tdtomato_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mCherry-Tandem-PSAM4-GlyR
Plasmid#208361PurposeExpression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.DepositorInsertmCherry-TANDEM-PSAM4-GlyR
UseAAVTagsmCherryExpressionMammalianPromoterCAGAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-9.47_VP2_AS #2562
Plasmid#74238PurposeModified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.DepositorInsertsAAV2 Rep
AAV9.47 VP2-AS
UseAAVAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS ChrimsonR mCherry_tr
Plasmid#111546PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-VenusYFP-3AT
Plasmid#71269PurposeEntry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGateway CloningTags4xGly linker and T3A pea Pisum sativum ribulose-1…Available SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-pNFkB::Cre-mRuby3
Plasmid#164137PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFaDepositorInsertNFkB promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…ExpressionMammalianPromoterNFkB synthetic promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAR-B1(1986.1)
Plasmid#78503PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVExpressionMammalianAvailable SinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO_Ollas.S.V5_mCherry-NLS
Plasmid#178211PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsNuclear Localization Signal and Ollas.S.V5PromotereF1aAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-AMPKalpha2 wt
Plasmid#31652DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
TagsM2ExpressionMammalianAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE GFP-Ago2 S387A
Plasmid#60654PurposeGFP-Ago2 S387A mutant lentiviral vectorDepositorAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGV3341
Plasmid#170279PurposeExpression of mutant HIV-2 RT p66 reverse transcriptaseDepositorInsertHIV-2 RT p66 (mutant)
ExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-3xVenusYFP-OcsT
Plasmid#71271PurposeEntry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsert3 times VenusYFP
UseGateway CloningTagsoctaline synthase terminatorAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
TagsHexahistidineExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS ChrimsonR mCherry_tr
Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-CDC27 wt
Plasmid#31673DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGV3319
Plasmid#170277PurposeExpression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)DepositorInsertBst-LF D720A
TagsHexahistidineExpressionBacterialMutationD720APromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
FCK-Chronos-GFP
Plasmid#80902PurposeLenti-mediated expression of Chronos-GFP under the CaMKII promoter.DepositorInsertChronos-GFP
UseLentiviralTagsGFPExpressionMammalianPromoterCaMKIIAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc-ns
Plasmid#226719PurposePlasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
TagsHA tagExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-AsCpf1(RR)
Plasmid#109325PurposeAAV vector expressing AsCpf1 (RR variant)DepositorInserthAsCpf1(RR)
UseAAVTagsHA and NLSMutationS542R/K607RPromoterhSyn1Available SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HSV.S_mCherry-NLS
Plasmid#178224PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HSV.S and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-AMPKalpha2 M93G
Plasmid#31653DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
TagsM2ExpressionMammalianMutationchanged Methionine 93 to GlycineAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only