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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,044 results for: cloning

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Showing: 581 - 600 of 63044 results

  7. pMOD_C3306

    Plasmid
    #91102
    Purpose
    Module C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    FMV 34S
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    91102-plasmid-map-sequence-id-202168

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  8. pMOD_A1010

    Plasmid
    #91030
    Purpose
    Module A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    ZmUbi
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91030-plasmid-map-sequence-id-181782

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  9. pMOD_A1002

    Plasmid
    #91029
    Purpose
    Module A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    AtUbi10
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91029-plasmid-map-sequence-id-181781

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  11. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid
    #129026
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129026-plasmid-map-sequence-id-256128

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  14. pMOD_B2000

    Plasmid
    #91059
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    none (begins with P2A to be fused to TALEN_1)
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91059-plasmid-map-sequence-id-181870

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  17. TETv4

    Plasmid
    #167983
    Purpose
    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Publication
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    TETv4 (TET1CD-XTEN80-dCas9)
    Use
    CRISPR and Synthetic Biology
    Tags
    3xNLS, TET1 catalytic domain, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    167983-plasmid-map-sequence-id-418937

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  19. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Publication
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159 Articles
    47549-plasmid-map-sequence-id-228099

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Showing: 581 - 600 of 63044 results