162,050 results

1. pPath-Violacein-native

   Plasmid

   #200001

   Purpose

   Violacein pathway. Native promoter and CDS sequence

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Pathways
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB-rDA3h

   Plasmid

   #208703

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-wtTnsAB

   Plasmid

   #234731

   Purpose

   Wild-type human-codon optimized PseTnsAB in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseTnsAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-wtTnsC

   Plasmid

   #234732

   Purpose

   Wild-type human-codon optimized PseTnsC in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseTnsC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. cyto-iATPSnFR1.0

   Plasmid

   #102550

   Purpose

   cytoplasmic ATP sensor for mammalian cells

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. synIRES_RC25

   Plasmid

   #189970

   Purpose

   Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   synIRES_RC25
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHTN-HaloTag_BCL11A:1-773

   Plasmid

   #211014

   Purpose

   Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   BCL11A:M1-F773 (BCL11A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   isoform 2

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Nathaniel Heintz, Alexander Nectow, Eric Schmidt

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C3_SEPT6

    Plasmid

    #71583

    Purpose

    mammalian expression of human SEPT6 with a GFP fusion

    Depositor

    Elias Spiliotis

    Article

    Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

    Insert

    Septin 6 (SEPTIN6 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    V206D

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_HEK3+1T>A_(PP7-C4-Q1)

    Plasmid

    #232437

    Purpose

    pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-AAVS1-PGK-Blast

    Plasmid

    #227270

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-EF1-DIO-tdTomato

    Plasmid

    #72254

    Purpose

    Express tdTomato under EF-1 promoter when Cre is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    tdTomato
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPomyces-2BD

    Plasmid

    #234428

    Purpose

    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified Cas9 with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5 beta1

    Plasmid

    #16042

    Depositor

    Filippo Giancotti

    Article

    Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

    Insert

    beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDIO-DSE-mCherry-PSE-MCS

    Plasmid

    #129669

    Purpose

    Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

    Depositor

    Beatriz Rico

    Article

    Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Rab35 S22N inactive

    Plasmid

    #47426

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 S22N (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S22N inactive

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. AcGFP1-C1

    Plasmid

    #54607

    Purpose

    Localization: C1 Cloning Vector, Excitation: 475, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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