167,318 results

1. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

   Plasmid

   #84243

   Purpose

   Expresses ABA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sa dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJPC596 FoMV-AmCyan

   Plasmid

   #234812

   Purpose

   To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   AmCyan
   

   Expression

   Plant

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HA-UBA6

   Plasmid

   #136995

   Purpose

   Expresses human UBA6 in mammalian cells

   Depositor

   Marcus Groettrup

   Article

   Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

   Insert

   UBA6 (UBA6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone

   Pooled Library

   #1000000083

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human kinome

   Depositor

   John Doench, David Root

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pVAX1/mTyr-Cre

   Plasmid

   #99249

   Purpose

   Expresses Cre recombinase under tyrosinase promoter

   Depositor

   Christian Blank

   Article

   Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

   Inserts

   Cre recombinase
   mTyr enh/prom
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFD3-dU6:3gRNA

   Plasmid

   #49410

   Purpose

   expression of gRNA under control of the Drosophila U6:3 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-3:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-3

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126706

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-opto-mCasp8

   Plasmid

   #208782

   Purpose

   Inducibly expresses mouse caspase8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mCaspase8 Cry2 (Casp8 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. DHFR-SpCas9-DHFR

   Plasmid

   #85447

   Purpose

   Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-SpCas9-DHFR
   

   Use

   AAV

   Tags

   Destabilized domain (C-terminus version) of E.col…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Wnt7B

    Plasmid

    #35915

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt7B (Wnt7b Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-HisMBP

    Plasmid

    #11085

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    Tac (lactose/IPTG inducible)

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKL2315

    Plasmid

    #187876

    Purpose

    Expresses Cre recombinase.

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    J23107

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBA615

    Plasmid

    #186239

    Purpose

    eLbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-LbuCas13a + crRNA negative control
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 765 pME nls mCerulean

    Plasmid

    #195958

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS165

    Plasmid

    #194488

    Purpose

    97-4 Op (Part 2)

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    97-4 Op (Part 2)
    

    Use

    Synthetic Biology

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-Sec16A

    Plasmid

    #36155

    Purpose

    Expresses Sec16A as a GFP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human USP7-3X FLAG

    Plasmid

    #225334

    Purpose

    Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

    Plasmid

    #192204

    Purpose

    Lenti-GL(GFP-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-GL(GFP-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

    Plasmid

    #100848

    Purpose

    AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1 and AAV9

    Insert

    jRCaMP1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. Strep-KIFC1*-mCh

    Plasmid

    #120169

    Purpose

    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member C1 (KIFC1 Human)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIFC1: 125-673 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. CLYBL-TO-hNIL-BSD-mApple

    Plasmid

    #124230

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Insert

    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Expression

    Mammalian

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCDH-hMALAT1

    Plasmid

    #118580

    Purpose

    to overexpress human MALAT1 in mammalian cells

    Depositor

    Jianjun Zhao

    Article

    Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

    Insert

    MALAT1 (MALAT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGEX-p47PX(aa2-132)

    Plasmid

    #19007

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p47phox (NCF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa2-132 (PX domains)

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pUAST-pHuji-GINKO2

    Plasmid

    #177117

    Purpose

    Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

    Depositor

    Robert Campbell

    Article

    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

    Insert

    pHuji-GINKO2
    

    Expression

    Insect

    Promoter

    hsp70 promoter

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pNeae2

    Plasmid

    #168300

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

    Insert

    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    

    Tags

    6xHis, E-tag, and myc-tag

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR223_NOTCH2_WT

    Plasmid

    #82292

    Purpose

    Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NOTCH2 (NOTCH2 Human)
    

    Use

    Gateway entry vector

    Mutation

    C946Y

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. nAChR beta2 WT

    Plasmid

    #24272

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR beta2 WT (Chrnb2 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET263-pUC57 24xMS2V7

    Plasmid

    #140705

    Purpose

    MS2V7 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V7
    

    Use

    Cloning vector

    Mutation

    24 MS2 loops V7, high-affinity C-variant, inter-s…

    Promoter

    None

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGEmCherry2

    Plasmid

    #118413

    Purpose

    Red fluorescent reporter for assessing CRISPR gRNA activity

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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