We narrowed to 11,723 results for: Vars

1. pCMV-RVR-A3A-N57G

   Plasmid

   #193657

   Purpose

   Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   RVR-dLbCas12a-hA3A-N57G, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-PDGFRA-V5/HIS

   Plasmid

   #234754

   Purpose

   Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF-Mm2

   Plasmid

   #197100

   Purpose

   To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   PylRS variant, Pyl tRNA, kanamycin resistance gene
   

   Expression

   Bacterial

   Mutation

   Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

   Plasmid

   #177669

   Purpose

   Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

   Depositor

   Karl Deisseroth

   Article

   Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   nEF

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-ERBB2

   Plasmid

   #175846

   Purpose

   Expresses ERBB2

   Depositor

   Evan Braunstein

   Article

   Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

   Insert

   ERBB2 (ERBB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2273

   Plasmid

   #111899

   Purpose

   ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple343 (TUBB3 Human)
   

   Use

   AAV

   Promoter

   Ple343 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_PKIA_WT

   Plasmid

   #82153

   Purpose

   Gateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PKIA (PKIA Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT4-U6-sgRNA-CMV-EGFP

   Plasmid

   #239587

   Purpose

   Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Type

   Empty backbone

   Use

   Sleeping beauty transposone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX303-PCDH7-a

    Plasmid

    #108538

    Purpose

    Expresses human PCDH7 transcript variant a in mammalian cells

    Depositor

    Kathryn O’Donnell

    Article

    Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

    Insert

    PCDH7 transcript variant a (PCDH7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_RHEB_WT

    Plasmid

    #81257

    Purpose

    Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 SARS-CoV-2 S D614G

    Plasmid

    #158075

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-HF1-plus

    Plasmid

    #126768

    Purpose

    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

    Plasmid

    #201032

    Purpose

    Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPGR-pTF-dnTCF4

    Plasmid

    #114277

    Purpose

    TET-inducible expression of a dominant negative form of the TCF4 transcription factor

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Mutation

    deletion of AA 1 to 31

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-YBX1

    Plasmid

    #192904

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    YBX1 (YBX1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106180

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV5

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-BLAST TFR1

    Plasmid

    #169890

    Purpose

    Express TFR1 open reading frame. Lacks IRE regulatory elements

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    TFRC (TFRC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    TFRC cDNA, lacking 3'UTR regulatory elements

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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