We narrowed to 8,836 results for: construct
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Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralExpressionMammalianPromotermU6 promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
human-GFAP-EGFP
Plasmid#169473PurposeWe generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.DepositorAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg30-PDGFR_EF1a_mCherry
Plasmid#186309PurposePlasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg30-PDGFR
mCherry
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPO2
Plasmid#209976PurposeContains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (SH71_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3E-MCS
Plasmid#75174PurposeMultiple cloning site for insertion of gene of interest for C-terminal fusions.DepositorInsertMultiple Cloning Site
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCh-kCAAX
Plasmid#112267PurposeITRACK Acceptor ConstructDepositorInsertmCherry with KRas membrane targeting domain
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPO1
Plasmid#209975PurposeContains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (256_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
LZF43: Cre-on CAG-CheRiff-CFP (DIO)
Plasmid#104114PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseLentiviralExpressionMammalianPromoterCAGAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR PGK puro
Plasmid#12084PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-COX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR
Plasmid#235203PurposePlasmids for constructing mitoBEs v2DepositorInsertCOX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR
ExpressionMammalianAvailable SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor lambda version
Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPPromoterFCP and U6Available SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-PuroTK-L2
Plasmid#141005PurposeMutisite Gateway mediated vector constructionDepositorInsertPuroTK
ExpressionMammalianAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
TagsHA, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-mAmetrine
Plasmid#18083PurposeBacterial expression of mAmetrine fluorescent proteinDepositorInsertmAmetrine fluorescent proteins
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G12-CheRiff-T2A-mCherry
Plasmid#170275PurposeCheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertCheRiff-T2A-mCherry
UseAAVPromoter11xUAS with TATA-box minimal promoterAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only