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pRG205

Plasmid

#64524

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRG205

Plasmid

#64524

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUltra-puro-RTTA3

Plasmid

#58750

Purpose

silence doxycyclin inducible pUltra-dox

Depositor

Yildirim Dogan, Kitai Kim

Insert

Reverse TET-Transactivator 3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

loxP5171

Promoter

Ubiquitin C promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pUltra-puro-RTTA3

Plasmid

#58750

Purpose

silence doxycyclin inducible pUltra-dox

Depositor

Yildirim Dogan, Kitai Kim

Insert

Reverse TET-Transactivator 3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

loxP5171

Promoter

Ubiquitin C promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRG206

Plasmid

#64527

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRG206

Plasmid

#64527

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (N154S)

Plasmid

#214160

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (N154S) (STING1 Human)

Use

Tags

Expression

Mammalian

Mutation

N154S

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-P4M-SidMx2

Plasmid

#221747

Purpose

Stable expression of GFP-P4M-SidMx2 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

P4M-SidM(546-647)x2

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG-FGF13A

Plasmid

#220793

Purpose

To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

Insert

FGF13 (FGF13 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Mango IV

Plasmid

#127584

Purpose

Expresses 5S-F30-Mango IV rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold and Mango IV tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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ATMW-BL21

Bacterial Strain

#218769

Purpose

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

Bacterial Resistance

Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

Species

Escherichia coli

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Use

Tags

MLALKLAGLDI

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Use

Tags

MLALKLAGLDI

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG3 Rab5CA

Plasmid

#78268

Purpose

Rab5 Constitutively active (Q79L) expression

Depositor

Felicia Goodrum

Insert

RAB5A (RAB5A Human)

Use

Lentiviral

Tags

IRES-EGFP and mCherry

Expression

Mammalian

Mutation

Q79L

Promoter

CMV, Lentiviral LTR

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG3 Rab5CA

Plasmid

#78268

Purpose

Rab5 Constitutively active (Q79L) expression

Depositor

Felicia Goodrum

Insert

RAB5A (RAB5A Human)

Use

Lentiviral

Tags

IRES-EGFP and mCherry

Expression

Mammalian

Mutation

Q79L

Promoter

CMV, Lentiviral LTR

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge