We narrowed to 1,391 results for: lentiviral backbone

1. pLenti GW-NL-myc

   Plasmid

   #113455

   Purpose

   Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   NanoLuc-cmyc

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti PA-mCit-GW

   Plasmid

   #113456

   Purpose

   Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. WPXL LEFTY2 gRNA

   Plasmid

   #101924

   Purpose

   LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   LEFTY2 enhancer targeting gRNA
   

   Use

   Lentiviral

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. WPXL SOX10 gRNA

   Plasmid

   #101923

   Purpose

   SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   SOX10 promoter targeting gRNA
   

   Use

   Lentiviral

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL.SSA TLR (Ani)

   Plasmid

   #45577

   Purpose

   Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with I-Ani I TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Ani I TS from 163-185bp, truncated 25 …

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-puro_Panc480-MT7

   Plasmid

   #200941

   Purpose

   Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   multiplexed sgRNA array
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, EF-1a

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

   Plasmid

   #189633

   Purpose

   Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

   Plasmid

   #189636

   Purpose

   Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

    Plasmid

    #157982

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

    Plasmid

    #123324

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

    Plasmid

    #123325

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIN-PAmCherry-mSNCA-NE

    Plasmid

    #102364

    Purpose

    Lentiviral overexpression of PA-mCherry synuclein alpha fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    synuclein alpha (Snca Mouse)
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIN-hSNCA-NE

    Plasmid

    #102366

    Purpose

    Lentiviral overexpression of synuclein alpha

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Insert

    synuclein alpha (SNCA Human)
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    A silent mutation (333bp downstream of ATG) was i…

    Promoter

    EF-1a

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLS-mP

    Plasmid

    #81225

    Purpose

    Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    Derived from pGL4.23

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVpuro-CMV-N-EGFP

    Plasmid

    #122848

    Purpose

    N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-EF1a-GFP1-10-IRES-Puro

    Plasmid

    #211467

    Purpose

    Lentivirus backbone expressing GFP1-10

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA

    Plasmid

    #164787

    Purpose

    pLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    1.3αCaMKII

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA

    Plasmid

    #164775

    Purpose

    pLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211462

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLB

    Plasmid

    #11619

    Purpose

    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

    Depositor

    Stephan Kissler

    Article

    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTS1106_Tier3(Lenti)

    Plasmid

    #169661

    Purpose

    Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-CMV-dsRed-UBC-GFP-W

    Plasmid

    #24526

    Depositor

    Darrell Kotton

    Article

    Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    DsRed and GFP

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BTV

    Plasmid

    #84771

    Purpose

    bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production

    Depositor

    David Erle

    Article

    Zhao et al Nat Biotechnol. 2014 Apr;32(4):387-91. d

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LMAPCW

    Plasmid

    #205655

    Purpose

    shRNAmir backbone for RNA interference, with mCherry reporter

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Lentiviral

    Promoter

    mCMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge