We narrowed to 6,241 results for: cas9 expression plasmid

1. Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility

   Pooled Library

   #101931

   Purpose

   Bassik lab Human CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility. Coexpresses mCherry.

   Depositor

   Michael Bassik

   Article

   Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

   Expression

   Mammalian

   Use

   CRISPR

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

2. Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

   Pooled Library

   #101927

   Purpose

   Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.

   Depositor

   Michael Bassik

   Article

   Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

   Expression

   Mammalian

   Use

   CRISPR

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

3. PAX6 CKO 2/7 Donor plasmid

   Plasmid

   #68467

   Purpose

   donor plasmid for targeting PAX6 locus

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   FRT flanked exon4 of PAX6 gene (PAX6 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

   Pooled Library

   #89640

   Purpose

   Paired sgRNA library containing 12,472 pgRNAs targeting 671 human long non-coding RNAs (lncRNAs)

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

5. Anti-VGlut3 [N34/34.2R] (Recombinant Antibody)

   Antibody

   #184216-rAb

   Purpose

   Anti-VGlut3 (Rat) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Immunocytochemistry and Immunohistochemistry

   Reactivity

   Mouse and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available since

   Aug. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

6. Jun lab tunable CRISPRi strain

   Bacterial Strain

   #86400

   Purpose

   Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

   Depositor

   Suckjoon Jun

   Article

   Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

   Bacterial Resistance

   Spectinomycin and hygromycin

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pITI2

   Plasmid

   #166095

   Purpose

   Gal inducible expression of D10A-Cas9 nickase

   Depositor

   Rebecca Burgess

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A

   Promoter

   Gal-inducible

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPH3

   Plasmid

   #166098

   Purpose

   Gal inducible expression of D10A-Cas9 nickase

   Depositor

   Rebecca Burgess

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A

   Promoter

   Gal-inducible

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

   Viral Prep

   #73178-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Promoter

   Tags

   None

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

10. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep

    #73633-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Promoter

    Tags

    None

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

11. AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

    Plasmid

    #68347

    Purpose

    AAV plasmid expressing the FlpO recombinase. with empty gRNA

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    FlPO
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFREE

    Plasmid

    #92050

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRR1

    Plasmid

    #166096

    Purpose

    Gal inducible expression of Cas9 WT

    Depositor

    Rebecca Burgess

    Insert

    WT spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFREE_Zeo

    Plasmid

    #92053

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJB06

    Plasmid

    #190480

    Purpose

    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Cas9
    XylR
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    xylR-driven promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP942-pX330-Sncg

    Plasmid

    #181774

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Sncg guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP926-pX330-Batf3

    Plasmid

    #181772

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Batf3 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AiP927-pX330-Chrna6

    Plasmid

    #181765

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Chrna6 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP928-pX330-Gpr139

    Plasmid

    #181767

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Gpr139 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AiP938-pX330-Slc17a7

    Plasmid

    #181775

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Slc17a7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AiP939-pX330-Ctxn3

    Plasmid

    #181769

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Ctxn3 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KO plasmid

    Plasmid

    #135970

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMK

    Plasmid

    #62818

    Purpose

    Bacterial constitutive S. pyogenes Cas9 expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJAT17

    Plasmid

    #204298

    Purpose

    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJCHph

    Plasmid

    #185863

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker

    Depositor

    Claudia Vickers

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJCKan

    Plasmid

    #185862

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker

    Depositor

    Claudia Vickers

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMZ379

    Plasmid

    #74215

    Purpose

    Combined sgRNA/Cas9 vector with NatMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMZ377

    Plasmid

    #74214

    Purpose

    Combined sgRNA/Cas9 vector with LEU2 marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMZ376

    Plasmid

    #74213

    Purpose

    Combined sgRNA/Cas9 vector with KanMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCJ1022

    Plasmid

    #228762

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Pkd2 gRNAs (Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid

    #154429

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiCRISPRv2_ZsG gRNA_4

    Plasmid

    #192688

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiCRISPRv2_ZsG gRNA_1

    Plasmid

    #192685

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lentiCRISPRv2_ZsG gRNA_2

    Plasmid

    #192686

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. lentiCRISPRv2_ZsG gRNA_3

    Plasmid

    #192687

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge