We narrowed to 24,827 results for: crispr

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pC0056 - LwCas13a-msfGFP-NES

Plasmid

#105815

Purpose

Expresses active LwaCas13a-NES for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NES and msfGFP-NES-3xHA

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

Plasmid

#60231

Purpose

Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase
EGFP

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA-P2A and EGFP-KASH

Expression

Mammalian

Promoter

U6 and hSyn

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pC035 - dLwCas13a-msfGFP

Plasmid

#91925

Purpose

Expresses catalytically inactive LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS-3xHA and NLS-msfGFP

Expression

Mammalian

Mutation

R488A and R1060A

Promoter

EF-1a

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-GFP

Plasmid

#132776

Purpose

Prime editing in mammalian cells with co-translational GFP expression

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

35Sp::hCas9

Use

CRISPR

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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lentiMPH v2

Plasmid

#89308

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Insert

MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ230 (LbCpf1)

Plasmid

#86210

Purpose

LbCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR; Gateway compatible lbcpf1 entry clone

Tags

5' and 3' NLS

Expression

Plant

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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