We narrowed to 8,785 results for: sgrna

1. pSLQ14103 aeBlue-targeting_sgRNA

   Plasmid

   #239750

   Purpose

   guide for aeBlue

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   guide targeting aeBlue
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDN-Cherry-sgRNA

   Plasmid

   #221387

   Purpose

   episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   none, cloning vector for introduction of targeting sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7h sgRNA for 4-μHOM reporter

   Plasmid

   #113625

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7h sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

4. LentiCRISPRv2 EGFP sgRNA1

   Plasmid

   #164932

   Purpose

   Expresses EGFP sgRNA1 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. L4440_BioBrick-hif-1-sgRNA-A

   Plasmid

   #177784

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti_sgRNA(MS2)_zsGreen1

   Plasmid

   #138463

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   zsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_DRS-1 sgRNA / hSpCas9

   Plasmid

   #172841

   Purpose

   Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_VCL sgRNA / hSpCas9

   Plasmid

   #172837

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWH1266-Apra-sgRNA

   Plasmid

   #218372

   Purpose

   sgRNA expression plasmid for CRISPR-intereference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   sgRNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB-BbsI-sgRNA

    Plasmid

    #203359

    Purpose

    Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    BbsI Cut Sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-U6-sgRNA-BFP

    Plasmid

    #107722

    Purpose

    For expression of sgRNA from the U6 promoter with BFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid

    #172836

    Purpose

    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid

    #172834

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. L4440_BioBrick-hif-1-sgRNA-B

    Plasmid

    #177785

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hif-1 (hif-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLH-sgRNA1-2XPP7

    Plasmid

    #75390

    Purpose

    sgRNA1-2XPP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_CALR sgRNA / hSpCas9

    Plasmid

    #172838

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCAR_sgRNA_hygro-tagBFP-lox5171

    Plasmid

    #162070

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid

    #181783

    Purpose

    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBx-Spas-sgRNA-Gm

    Plasmid

    #105232

    Purpose

    Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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