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pTHSSd_27

Plasmid

#59958

Purpose

PTac null fragment (sigma CGG Y639A) expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

null fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGL0_19 [panARS ORI]

Plasmid

#198932

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

panARS ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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lentiGuide-mMaroon

Plasmid

#217008

Purpose

lentiviral plasmid for gRNA cloning with selectable mMaroon expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-CLIPf-WPRE

Plasmid

#104107

Purpose

Expresses CLIPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

CLIPf

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAct1IsGFP-1

Plasmid

#38775

Depositor

Peggy Lemaux

Article

Cho et al Plant Cell Rep. 2000 Nov;19(11):1084-108

Insert

Actin promoter and intron

Use

Plant transformation

Tags

nos 3' terminator and sGFP (S65T)

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Tags

3xFlag and myc

Expression

Bacterial

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pART (pT-2)

Plasmid

#62708

Purpose

Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pC23-LIC-His-FLAG

Plasmid

#165477

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCY 3080-01

Plasmid

#36226

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEmCitrine

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-kanMX

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR-221-P3-P5

Plasmid

#186350

Purpose

Gateway donor vector with AttP3 AttP5 recombination sites

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway donor vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-23

Plasmid

#57748

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vinculin (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLOX-EWgfp

Plasmid

#12242

Depositor

Didier Trono

Article

Salmon et al Blood. 2000 Nov 15. 96(10):3392-8.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

EF1a promoter is upstream of GFP. LoxP in 3'…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SSTR3-N-17

Plasmid

#55326

Purpose

Localization: Cilium, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SSTR3

Tags

mTagBFP2

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLV-EgI-NeoR

Plasmid

#207175

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Avi-tag

Plasmid

#117128

Purpose

C-terminal Tev-Avi tag Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev Avi-tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev Avi-Tag

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_CMVtrunc_877

Plasmid

#105828

Purpose

A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Type

Empty backbone

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-ED4

Plasmid

#193429

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBXNH3A

Plasmid

#47075

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pNC-121-35S

Plasmid

#193397

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCS6-mPSD95a-TS2-HA

Plasmid

#42240

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS2-HA

Expression

Mammalian

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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