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pFBOH-MHL.RBBP4

Plasmid

#125173

Purpose

Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Histone-binding protein RBBP4 (RBBP4 Human)

Tags

His-TEV site

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pR I-Ab alpha BirA

Plasmid

#113638

Purpose

Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab alpha BirA (H2-Ab1 Mouse)

Tags

BirA biotinylation signal site and fused to Fos-J…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pMXs-puro HA-Atg14*

Plasmid

#38265

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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(383) pcDNA3.1-HA-nBC2-OGA(544-706)

Plasmid

#168100

Purpose

HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nBC2-OGA(544-706)

Tags

HA-tag

Expression

Mammalian

Mutation

OGA truncated to contain residues 544-706

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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PH-Btk-mRuby2

Plasmid

#124657

Purpose

PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

BTK (BTK Human)

Tags

mRuby2

Expression

Mammalian

Mutation

contains only the PH domain of Bruton tyrosine ki…

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

Plasmid

#91885

Purpose

Retroviral expression of mouse Ezh2 with a SET domain deletion

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

SET domain deleted

Promoter

MMLV gag

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (IFT88, donor)

Plasmid

#80769

Purpose

Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

IFT88 gRNA#1 (IFT88 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 R67S

Plasmid

#53172

Purpose

Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Mustard Weed, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pLNCX1 myr Akt1 delta4-129

Plasmid

#15989

Depositor

Morris Birnbaum

Article

Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Delta 4-129

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pIRES proHB-EGF CS

Plasmid

#11603

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Mutation

Constitutively secreted HB-EGF: C-terminal amino …

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-EF1α1.1-GFP (AAV2)

Viral Prep

#122100-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-EF1α1.1-GFP (#122100). In addition to the viral particles, you will also receive purified pAAV-EF1α1.1-GFP plasmid DNA. EF1a-driven expression of GFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Promoter

EF1a（1.1kb short version)

Tags

GFP

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only
pYPQ141D

Plasmid

#69293

Purpose

Gateway entry vector; single gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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YCpLac111

Plasmid

#53249

Purpose

contains a single replication origin (ARS1) used for plasmid replication assay

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pSpdCas9-huTET1CD-T2A-mCherry(PX458)

Plasmid

#129027

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKan-PRFA1-9myc-AID*(N)

Plasmid

#99528

Purpose

N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

RFA1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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