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pLenti_CMV:Flag-dKeNLuc_SV40:PuroR

Plasmid

#183046

Purpose

Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

dKeNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-KaNLuc_SV40:PuroR

Plasmid

#183047

Purpose

Expresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

KaNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSB4k5_RNAnano (r-plasmid)

Plasmid

#127634

Purpose

Encodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

Insert

RNA nanostrcutre

Use

Synthetic Biology

Promoter

T7 RNAP

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-mdlC

Plasmid

#134590

Purpose

Low Phosphate inducible mdlC benzylformate decarboxylase

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

mdlC (benzylformate decarboxylase) C -terminal his tag

Tags

6x-His

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64TS human TGFbeta-IIR (DM#169)

Plasmid

#15012

Depositor

Douglas Melton

Article

Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

Insert

TGF beta type II receptor (TGFBR2 Human)

Use

Sp6 expression for use in xenopus

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pET20b-CeNLuc-6xHis

Plasmid

#135933

Purpose

Expresses the CeNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Promoter

cpc operon

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-92b

Plasmid

#22709

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro-RNA-92b (mir-92b Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DAZL

Plasmid

#222525

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DAZL (DAZL Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-BRCA1

Plasmid

#222530

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BRCA1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB1

Plasmid

#222534

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB1 (HMGB1 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HESX1

Plasmid

#222535

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HESX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HESX1 (HESX1 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RNF138

Plasmid

#222536

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RNF138

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RNF138 (RNF138 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOXB5

Plasmid

#222537

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXB5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-HOXB5-DD-hygro

Plasmid

#222582

Purpose

PiggyBac transposon plasmid for Shield1-inducible expression of BCL2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-HOXB5

Plasmid

#222584

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXB5, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-UBB

Plasmid

#222569

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of UBB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

UBB (UBB Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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