Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 22,620 results for: his

Clear keywords
Showing: 6341 - 6360 of 22620 results

  4. pHLmMBP-1

    Plasmid
    #72343
    Purpose
    Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72343-plasmid-map-sequence-id-132066

    Enlarge

  5. dCas9-FOG1[N+C]

    Plasmid
    #100085
    Purpose
    dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    FOG1(aa 1-45) (ZFPM1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-FOG1-dCas9-FOG1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100085-plasmid-map-sequence-id-197489

    Enlarge

  6. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Plasmids for integrating into well-characterized chromosomal loci to program gene expression in S. cerevisiae.
    Depositor
    Aindrila Mukhopadhyay
    Application
    Genome Editing
    Vector Type
    Yeast Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162685-plasmid-map-sequence-id-320886

    Enlarge

  11. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid
    #106309
    Purpose
    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.
    Depositor
    Zhe J. Liu
    Article
    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p
    Insert
    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    Tags
    6xflag-3xHaloTag(MtoL)
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106309-plasmid-map-sequence-id-204684

    Enlarge

  13. pACEC3GH (Hyg)

    Plasmid
    #110084
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110084-plasmid-map-sequence-id-211189

    Enlarge

  19. pET-LiCre

    Plasmid
    #219797
    Purpose
    Expresses a codon-optimized His-tagged Light-inducible Cre (LiCre) recombinase in E. coli. Plasmid ID in Yvert lab is pGY611. LiCre is described in https://doi.org/10.7554/eLife.61268
    Depositor
    Gaël Yvert
    Article
    Dufour et al Biol Open. 2026 May 15;15(5):bio062381.
    Insert
    Codon-optimized Light-inducible Cre Recombinase
    Use
    Cre/Lox
    Tags
    HIS-tag for purification
    Expression
    Bacterial
    Promoter
    IPTG-inducible
    Available Since
    May 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    219797-plasmid-map-sequence-id-437464

    Enlarge

Showing: 6341 - 6360 of 22620 results