165,188 results

1. pET19b-rDAD2 cPGFP(G164)

   Plasmid

   #165994

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rDAD2 cPGFP(G164)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-puro HGF

   Plasmid

   #10901

   Depositor

   Bob Weinberg

   Article

   Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

   Insert

   HGF (HGF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SMN2E7-Fluc-Nluc-reporter

   Plasmid

   #221005

   Purpose

   Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   Fluc-SMN2E7-STOP-Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG_GPHN.FingR-mKate2-IL2RGTC

   Plasmid

   #46297

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. spNW100

   Plasmid

   #173488

   Purpose

   Expresses spNW100 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW100
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   P1049L- please see depositor comments

   Promoter

   T7

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHEN2-SG4 R105A

   Plasmid

   #196072

   Purpose

   Express mutant (R105A) SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4 R105A
   

   Use

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Mutation

   R105A mutation in SG4

   Promoter

   T7

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-gp32-H6

   Plasmid

   #163912

   Purpose

   Expresses T4 gp32 for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   gp32
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.3-NL63-D14

   Plasmid

   #172666

   Purpose

   encodes NL63 spike protein, for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
   

   Use

   Lentiviral and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   c-terminal deletion of 14aa

   Promoter

   CMV

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM468-EGFP

    Plasmid

    #108434

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-NLS-GFP (AAV PHP.eB)

    Viral Prep

    #104061-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    CAG

    Tags

    GFP

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

12. EC70202

    Plasmid

    #209463

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU6

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid

    #183532

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. iLID-GFP-GCN4-ppKin14

    Plasmid

    #240420

    Purpose

    -end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    ppKin14Vib
    

    Use

    Tags

    GCN4, GFP, and iLID

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-KIF5A-FRB

    Plasmid

    #240425

    Purpose

    +end oriented kinesin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Vim-mCh-FKBP

    Plasmid

    #240423

    Purpose

    Tagged vimentin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KIF5A-mVenus-iLID

    Plasmid

    #240422

    Purpose

    +end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    iLID and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Vim-mCh-SspB

    Plasmid

    #240421

    Purpose

    Tagged vimentin for optogenetic vimentin pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    SspB and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRB-BFP-GCN4-ppKin14

    Plasmid

    #240426

    Purpose

    -end oriented kinesin for rapalog induced pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    ppKin14Vib
    

    Use

    Tags

    FRB, GCN4, and tagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmBaoJin-Actin

    Plasmid

    #229609

    Purpose

    Expresses mBaoJin-actin fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQAT11

    Plasmid

    #223383

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. tagRFP-GPHN.FingR-PhoCl

    Plasmid

    #236183

    Purpose

    Half of photoactivatable GFE3. When co-expressed with PBP-E3-HA (Plasmid #236182), ablates inhibitory synapses after exposure to 400 nm light.

    Depositor

    Don Arnold

    Article

    Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

    Insert

    tagRFP-GPHN.FingR-PhoCl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL4 [luc2P/Gli-RE/Hygro] Vector

    Plasmid

    #236803

    Purpose

    Express Gli response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    Gli (GLI1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    minP

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pYPQAT60

    Plasmid

    #223432

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF1639

    Plasmid

    #144370

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PSIP1 (PSIP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQAT58

    Plasmid

    #223430

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQAT61

    Plasmid

    #223433

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQAT08

    Plasmid

    #223380

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP_SeV_RdRP

    Plasmid

    #228808

    Purpose

    Expression of Sendai virus RdRP

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    RdRP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLentiCRISPRpuro-sgROSA26

    Plasmid

    #231562

    Purpose

    pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

    Depositor

    Rizwan Haq

    Article

    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

    Insert

    sgROSA26
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF-1a

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge