We narrowed to 858 results for: PX330

1. p330 VQR

   Plasmid

   #101730

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. C-12-BE

   Plasmid

   #135040

   Purpose

   C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjCDA1L2_1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. N-8-BE

   Plasmid

   #135023

   Purpose

   C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LpCDA1L1_1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. C-1-BE

   Plasmid

   #135029

   Purpose

   C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   PmCDA1L1_4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. N-4-BE

   Plasmid

   #135019

   Purpose

   C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjCDA1L1_4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLHDR_R4

   Plasmid

   #124211

   Purpose

   gRNA/Cas9 expression for 2nd editing using pSLHDR04

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C-13-BE

   Plasmid

   #135041

   Purpose

   C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LpCDA1L1_4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C-7-BE

   Plasmid

   #135035

   Purpose

   C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LpCDA1L1_1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C-6-BE

   Plasmid

   #135034

   Purpose

   C-T base editing using LjDA1L2_2 fused to C terminal of nCas9

   Depositor

   Tian-Lin Cheng, Zilong Qiu

   Article

   Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

   Insert

   LjDA1L2_2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 with EF-1alpha intron

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C-11-BE

    Plasmid

    #135039

    Purpose

    C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

    Depositor

    Tian-Lin Cheng, Zilong Qiu

    Article

    Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

    Insert

    LpCDA1L2_2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 with EF-1alpha intron

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLHDR_R1

    Plasmid

    #124209

    Purpose

    gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Halo-TCAB1 KI sgRNA

    Plasmid

    #207611

    Purpose

    sgRNA for HaloTag insertion into the endogenous TCAB1 locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    CCAAAGTCTTCATACTGCAG
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7b sgRNA for EJ7-GFP reporter

    Plasmid

    #113624

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7b sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

14. 7h sgRNA for 4-μHOM reporter

    Plasmid

    #113625

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7h sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

15. 7i sgRNA for 4-μHOM reporter

    Plasmid

    #113626

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7i sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

16. N-terminal Halo-XLF

    Plasmid

    #207546

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER50-SpCas9-ER50

    Plasmid

    #85448

    Purpose

    Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    ER50-SpCas9-ER50
    

    Use

    AAV

    Tags

    destabilized domain of estrogen receptor ligand b…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pREDIT_Cas9-MS2-BB_BbsI

    Plasmid

    #164802

    Purpose

    REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

20. N-Terminal Halo XRCC4

    Plasmid

    #207541

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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