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pDONR223-TRPM7

Plasmid

#23476

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRPM7 (TRPM7 Human)

Use

Gateway Cloning

Mutation

Insert encodes NCBI: NR_149154.2 bases 265 to 146…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK1

Plasmid

#23498

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway Cloning

Mutation

missing first codon

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hAtg101

Plasmid

#22876

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

Atg13-interacting protein (ATG101 Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP_PCNA_IRES_puro2b

Plasmid

#21048

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

hPCNA

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only

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pCIG-RARa-403-myc [TJ#344]

Plasmid

#16286

Depositor

Thomas Jessell

Article

Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

Insert

RAR alpha 403 (RARA Human)

Tags

myc

Expression

Mammalian

Mutation

403 truncation. Dominant repressor derivative.

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
shLuc pRRL

Plasmid

#19125

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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PL-452 N-EGFP

Plasmid

#19173

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

EGFP

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCEP4 Bcl-2

Plasmid

#16461

Depositor

Bert Vogelstein

Article

Pietenpol et al Cancer Res. 1994 Jul 15. 54(14):3714-7.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pTSA-C

Plasmid

#17329

Depositor

Takeshi Sano

Article

Reznik et al Bioconjug Chem. . 12(6):1000-4.

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
V1664 pLPS-3'RFP-mito - Acceptor

Plasmid

#11702

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP-mito

Expression

Mammalian

Available Since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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HA GST p85 S6K1 pRK5

Plasmid

#8466

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
scAAV-sgRNA-GFP

Plasmid

#177935

Purpose

Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRB

Plasmid

#116770

Purpose

Lentiviral expression of PDGFRB

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRB (PDGFRB Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK6

Plasmid

#116725

Purpose

Lentiviral expression of CDK6

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pMB66

Plasmid

#47946

Purpose

Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

Expression

Worm

Promoter

hsp-16.48

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCK017 pDEST exorh:EGFP

Plasmid

#195983

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

aa990-1912

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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