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pRK5-FLAG-Sestrin2-L261A

Plasmid

#73671

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNA-R-gfp

Plasmid

#220927

Purpose

Expresses gfp using J23119 promoter and pgRNA_AT backbone

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

superfolder GFP

Tags

FLAG

Expression

Bacterial

Promoter

J23119

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available Since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGEM-SNAP-TRIM21

Plasmid

#196181

Purpose

For in vitro transcription of SNAP-TRIM21 mRNA

Depositor

Binyam Mogessie

Article

Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

Insert

SNAP-TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMIGII-TCR-E

Plasmid

#194391

Purpose

Retroviral expression of candidate orphan TCR

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

TCR-E (Tcra, Tcrb Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJM585 ZF6x6-C mKate2 in TUPV1

Plasmid

#161529

Purpose

Inducible expression of mKate2 under the ZF6x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF6x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Promoter

PhmtB

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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JAB2240

Plasmid

#190359

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::PhlF-NLS-19Sterm

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5-Hygro

Plasmid

#195510

Purpose

lentiviral vector expressing RAP2A CDS with hygromycin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Hygromycin

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRep

Plasmid

#199599

Purpose

Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

Depositor

George Church

Article

Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

Insert

AAV2 rep

Use

AAV

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCeruleanBb

Plasmid

#118235

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCerulean

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pIA033

Plasmid

#200420

Purpose

thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

bsPAmCherry

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate10

Plasmid

#165007

Purpose

Expression of candidate TCR #10 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDN-LexAOx4-mNeonGreen

Plasmid

#192604

Purpose

Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mNeonGreen

Use

Unspecified

Promoter

Minimal promoter

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Expression

Bacterial

Promoter

T7

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-HIS-ybbr

Plasmid

#101238

Purpose

SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

SdrG_N2N3

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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