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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,392 results for: crispr grnas

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Showing: 6401 - 6420 of 8392 results

  1. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  2. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  7. LentiGuide-10X-CS1-3'-puro

    Plasmid
    #212011
    Purpose
    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)
    Depositor
    Danwei Huangfu
    Insert
    gRNA scaffold
    Use
    Lentiviral
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212011-plasmid-map-sequence-id-419682

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  10. pV1090

    Plasmid
    #111427
    Purpose
    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111427-plasmid-map-sequence-id-212460

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  11. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  20. pSCBE3

    Plasmid
    #133966
    Purpose
    Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Zhong et al bioRxiv 630137
    Insert
    BE3
    Use
    CRISPR and Synthetic Biology
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    133966-plasmid-map-sequence-id-266621

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Showing: 6401 - 6420 of 8392 results