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We narrowed to 66,568 results for: des.1

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Showing: 6461 - 6480 of 66568 results

  2. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  3. pNO286-3

    Plasmid
    #107746
    Purpose
    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
    Depositor
    Jeffrey Tabor
    Article
    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb
    Inserts
    CcaS
    Ho1
    PcyA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Deleted AA 223 and 512
    Promoter
    J23106 and J23108
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107746-plasmid-map-sequence-id-209202

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  7. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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  10. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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  11. pVITRO1-102.1F10-IgE/λ

    Plasmid
    #50365
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Expression
    Mammalian
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50365-plasmid-map-sequence-id-367944

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  19. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  20. mApple-Homer1-C-18

    Plasmid
    #54912
    Purpose
    Localization: Membrane Adaptor, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Homer1 (Homer1 Rat)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6461 - 6480 of 66568 results