We narrowed to 35,396 results for: CAL

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V72 ELP

Plasmid

#68938

Purpose

Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

Depositor

Andrew MacKay

Article

Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

Insert

V72 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMito-MtrxRFP2

Plasmid

#172324

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Tags

His-tag and Mitochondria localization sequence

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-fCAMK-GFP

Plasmid

#22917

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-mUbp43-His

Plasmid

#12458

Depositor

Dong-Er Zhang

Article

Malakhov et al J Biol Chem. 2002 Mar 22. 277(12):9976-8

Insert

UBP43 (Usp18 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pMF-TetTight

Plasmid

#193288

Purpose

Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

rtTA-P2A-tTS

Use

Lentiviral

Expression

Mammalian

Promoter

EF‐1α

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CAG_3C-Twin-Strep

Plasmid

#113901

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CAG

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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FRB-TagRFPT-CAAX

Plasmid

#87710

Purpose

FRB-tagged TagRFPT; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FRB-TagRFPT-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-clathrin

Plasmid

#136563

Purpose

Clathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703-clathrin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-I997A

Plasmid

#111832

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A) (VCL Chicken)

Use

Lentiviral

Mutation

changed vinculin isoleucine 997 to alanine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1pr_v2-RT

Plasmid

#97088

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1pr_v2-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3.2-Clathrin-15

Plasmid

#57452

Purpose

Localization: Clathrin Vesicles, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Article

Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

Insert

Clathrin (CLTB Human)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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human p53-(1-393)-N268D

Plasmid

#24861

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Asparagine 268 mutated to Aspartate

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-GalT-IRES-Puromycin

Plasmid

#134862

Purpose

Expresses EGFP-tagged Golgi marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

beta 1,4-galactosyltransferase (B4GALT1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 83-398

Promoter

EF1a

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK131] pCOLADuet p14|MP1

Plasmid

#136145

Purpose

bacterial expression of p14 and MP1

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

p14 and MP1

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRARE-MBP-DEST

Plasmid

#84650

Purpose

Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

Depositor

Neil Olszewski

Article

Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

tac

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12.D11

Plasmid

#221151

Purpose

cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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IGF2BP1

Plasmid

#155676

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

IGF2BP1 (IGF2BP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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