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pXN-attPGal4LWL

Plasmid

#26261

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pCOCK CMVwt

Plasmid

#46559

Purpose

Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

Depositor

Clifford Wang

Article

Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

Insert

CMV wt promoter

Use

Synthetic Biology

Expression

Mammalian

Mutation

Our version has a G at base 724 (reference has C)

Promoter

CMV

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only

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UAS-Syt::7xHalo7

Plasmid

#87650

Purpose

For creating drosophila transgenics expressing UAS-Syt::7xHalo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::7xHalo7

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_227_Venus_173

Plasmid

#39835

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_105_Venus_195

Plasmid

#39821

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pOCC5

Plasmid

#118863

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x4

Plasmid

#63598

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX-tetO-Pcyc1-3xFLAG

Plasmid

#41021

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRGD-TcR

Plasmid

#74110

Purpose

Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

tetA(C)

Use

Bacterial vector based on pmb1 replicon

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD

Plasmid

#190089

Purpose

Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-GAL4DBD

Plasmid

#26256

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

ZIP- Gal4DBD (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT

Plasmid

#32374

Depositor

Carmen Williams

Article

Igarashi et al Dev Biol. 2007 Dec 1;312(1):321-30. Epub

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Puro Empty (w609-1)

Plasmid

#20957

Purpose

3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK7

Plasmid

#122676

Purpose

Expresses mouse PCSK7 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-3XFLAG

Plasmid

#105784

Purpose

Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-3XFLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-MBP-TEV

Plasmid

#105771

Purpose

Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

MBP-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKSV7

Plasmid

#26686

Depositor

Philip Youngman

Article

Smith et al Biochimie. . 74(7-8):705-11.

Type

Empty backbone

Available Since

Dec. 16, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBWB294

Plasmid

#140636

Purpose

pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pREDSIX

Plasmid

#74103

Purpose

mCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Type

Empty backbone

Use

Conjugative bacterial vector

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSUMO-YHRC

Plasmid

#54336

Purpose

Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

Depositor

Claes Andréasson

Article

Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

Type

Empty backbone

Tags

6His-SUMO (yeast gene: SMT3)

Expression

Bacterial

Promoter

T7-LacO

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pIC291

Plasmid

#51403

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska1 IMAGE 3909951 (SKA1 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-TRP1

Plasmid

#41601

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

13Myc and yeast ADH1 terminator

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-GAL4AD

Plasmid

#26255

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pGGD000

Plasmid

#48859

Purpose

Empty entry vector for creating GreenGate C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-TRP1

Plasmid

#41597

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (S65T) and yeast ADH1 terminator

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pegRNA2_tevopreQ1

Plasmid

#212039

Purpose

Bacterial expression of the pegRNA for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

pegRNA

Use

CRISPR

Promoter

J23119

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PPP1CB-miRFP703

Plasmid

#178524

Purpose

Expresses PP1c-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

PP1c-miRFP703 (PPP1CB Synthetic, Human)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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