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pMA_Auxiliary 1

Plasmid

#102709

Purpose

Provides the Middle-to-End linker 1 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 2

Plasmid

#102710

Purpose

Provides the Middle-to-End linker 2 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 3

Plasmid

#102711

Purpose

Provides the Middle-to-End linker 3 which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4A

Plasmid

#102712

Purpose

Provides the End-to-End linker 4A which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Γ

Plasmid

#102714

Purpose

Provides the End-to-End linker 4Γ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4Δ

Plasmid

#102715

Purpose

Provides the End-to-End linker 4Δ which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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p249_LTJ_1kbFoxp3wtTemplate

Plasmid

#82664

Purpose

1kb HDR template for murine wildtype Foxp3

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine wildtype Foxp3

Use

Hdr template for gene editing

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p243_LTJ_4kbCD45.1Template

Plasmid

#82663

Purpose

4kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

4kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p250_LTJ_2kbFoxp3wtTemplate

Plasmid

#82665

Purpose

2kb HDR template for murine wildtype Foxp3

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine wildtype Foxp3

Use

Hdr template for gene editing

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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SELENOM SXXS

Plasmid

#86580

Purpose

Bacterial expression of SELENOM SXXS for protein production

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

SELENOM (SELENOM Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

Sigal peptide was deleted; SXXS

Promoter

Tac

Available Since

April 5, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pJK_proNR2_LGK.1

Plasmid

#81161

Purpose

Expresses levoglucosan kinase variant LGK.1 with promoter proNR2

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase

Tags

His6x

Expression

Bacterial

Mutation

LGK.1 (L140I,S142A,A373C)

Promoter

proNR2

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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EYK346

Plasmid

#69514

Purpose

pTET pduD1-224

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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EYK345

Plasmid

#69513

Purpose

pTET pduD21-224

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Mutation

A206K

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIB empty (w335-1)

Plasmid

#17487

Purpose

Retroviral Empty control vector, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Expression

Mammalian

Promoter

CMV and EF1alpha

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HA-hNPHP1

Plasmid

#122872

Purpose

Expresses HA-tagged human NPHP1 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Hoque et al Reproduction. 2021 Jul 8;162(2):129-139.

Insert

human NPHP1 (NPHP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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LexAop2-myr::4xSNAPf

Plasmid

#87638

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cre

Plasmid

#198281

Purpose

Expresses Cre in mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Cre

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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mUAV

Plasmid

#102680

Purpose

Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:amilCP:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NLS (Nucleoplasmin)

Plasmid

#220342

Purpose

Mammalian expression vector BS2 in nucleus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NLS (Nucleus localization sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSLTS

Plasmid

#59386

Purpose

Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Type

Empty backbone

Use

Genome engineering

Expression

Bacterial

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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