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pcsII-MECL1-flag

Plasmid

#86774

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

immunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

unknown

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-LOC57019

Plasmid

#34811

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced apoptosis inhibitor 1 (CIAPIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV_Anc689-NG-nCas9

Plasmid

#163527

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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YES1 Y537F

Plasmid

#51299

Purpose

Expression of YES1 Y537F

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Mutation

Y537F

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-eBE

Plasmid

#107688

Purpose

Expresses dLbCpf1-eBE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-eBE

Expression

Mammalian

Mutation

dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Numb-mCherry

Plasmid

#174193

Purpose

Expresses Numb in mammalian cells

Depositor

Cheng-han Yu

Article

Yu et al Nat Commun. 2015 Oct 28;6:8672. doi: 10.

Insert

Numb (Numb Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Contains Numb aa 8-593

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

Plasmid

#216531

Purpose

Targeting vector for CRISPRd generation

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

ABI-dCas9-mCherry

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-WPRE

Plasmid

#130992

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGM117

Plasmid

#220168

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

CISD1 (CISD1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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TagBFP2-INPP4B-CAAX

Plasmid

#116857

Purpose

plasma membrane-targetted PI(3,4)P2 4-phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

HRAS(172-189) and TagBFP2

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-CHAMP1

Plasmid

#159892

Purpose

Expresses CHAMP1 (CAMP) in mammalian cells

Depositor

Kozo Tanaka

Article

Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

Insert

CHAMP1 (CHAMP1 Human)

Tags

pEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-indRecA56

Plasmid

#102294

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Use

CRISPR

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Blast-H2BC11

Plasmid

#207762

Purpose

Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PCMV-intron myc Rab6WT

Plasmid

#46781

Purpose

expression of WT Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 (RAB6A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

Plasmid

#232244

Purpose

Mammalian expression of N581K RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

N581K RLIM-GFP-IRES-mCherry (RLIM Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

N581K

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1[degron]-IRES-mCherry

Plasmid

#232240

Purpose

Mammalian expression of REX1[degron]-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

REX1 (aa 70-85)

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

Plasmid

#90015

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 CARD-FLAG WT

Plasmid

#163978

Purpose

Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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