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KAT5 sgRNA1

Plasmid

#138187

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT5

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT5 (KAT5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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KAT5 sgRNA2

Plasmid

#138188

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT5

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT5 (KAT5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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KAT2A sgRNA2

Plasmid

#138186

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT2A (KAT2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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KAT2A sgRNA1

Plasmid

#138185

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT2A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT2A (KAT2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

Plasmid

#163138

Purpose

Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

PLK1 (PLK1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) EGFP-fusion

Plasmid

#8535

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains eGFP fused to N-terminal of HOXB6 at AA…

Promoter

CMV

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pSBtet-6xHis-TFIIB-V5-BB

Plasmid

#242714

Purpose

Doxycycline-inducible expression of double-tagged 6xHis-TFIIB-V5 (human). Construct is resistant to Dharmacon OnTarget and Accell GTF2B siRNA pools.

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

Transcription Factor IIB (GTF2B Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

Construct is resistant to Dharmacon Accell and On…

Promoter

Tight TRE promoter

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP-TFIIB(D207A)-2xStrep

Plasmid

#213920

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(D207A)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(D207A)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) D207A (2) Coding region changed for resistanc…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP-TFIIB(S65A)-2xStrep

Plasmid

#213921

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65A)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(S65A)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) S65A (2) Coding region changed for resistance…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP-TFIIB(S65E)-2xStrep

Plasmid

#213922

Purpose

Sleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65E)

Depositor

Britt Glaunsinger

Article

Gulyas et al Genes Dev. 2026 May 13. doi: 10.1101/gad

Insert

TFIIB(S65E)-2xStrep (GTF2B Human)

Tags

2xStrep

Expression

Mammalian

Mutation

(1) S65E (2) Coding region changed for resistance…

Promoter

EF-1EF1a/RPBSA

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_3x_U6_Ctnnb1_CAG_nlsTagBFP2

Plasmid

#247933

Purpose

Targeting Ctnnb1 gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Article

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

Ctnnb1 sgRNAs (Ctnnb1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_3x_U6_Ikbkg_CAG_nlsTagBFP2

Plasmid

#247934

Purpose

Targeting Ikbkg gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Article

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

Ikbkg sgRNAs (Ikbkg Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_3x_U6_mmp25_CAG_nlsTagBFP2

Plasmid

#247935

Purpose

Targeting mmp25 gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Article

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

mmp25 sgRNAs (Mmp25 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_3x_U6_Slc2a1_CAG_nlsTagBFP2

Plasmid

#247936

Purpose

Targeting Slc2a1 gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Article

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

Slc2a1 sgRNAs (Slc2a1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pKG03549_pLentiX1-EFS-rtTA-P2A-mGL-WPRE-TRE-mRuby2-bGH

Plasmid

#252556

Purpose

Inducible Tet all-in-one circuit with upstream tandem syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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