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zgc:111821_L (OZ597)

Plasmid

#35237

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:111821 (zgc:111821 Zebrafish)

Use

Zebrafish targeting

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CACNA1F_R (OZ508)

Plasmid

#27183

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cacna1f (cacna1f Zebrafish)

Use

Zebrafish targeting

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
P25: pHR-SFFVp-NLS-FenixS-EGFP-FTH1

Plasmid

#250531

Purpose

Self-assebled 24-mer tagged with NLS, FenixS, and EGFP. Upon red-light activation can bind up to 24 Ash1-modified proteins.

Depositor

Andrew Woolley

Publication

Le et al bioRxiv 2025.11.29.691301

Insert

FenixS

Use

Lentiviral

Tags

EGFP-FTH1 and NLS

Expression

Mammalian

Promoter

SFFV

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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P30: FenixS-VP16-NLS-IRES-E-Ash1

Plasmid

#250553

Purpose

Mammalian expression of FenixS-VP16-NLS and E-Ash1, optogenetic system for far red-light control transcriptional activation

Depositor

Andrew Woolley

Publication

Le et al bioRxiv 2025.11.29.691301

Inserts

FenixS
Ash1

Tags

E protein and VP16-NLS

Expression

Mammalian

Promoter

SV40

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

Plasmid

#239609

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Publication

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1/7SKhybrid-filler

Use

CRISPR and Lentiviral

Available since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPB12A

Plasmid

#210035

Purpose

Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

Depositor

Michael Talkowski

Publication

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas12a
rtta-T2A-puro

Use

CRISPR

Tags

nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

Plasmid

#218344

Purpose

Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJPT002-M-HaloTag-1x FLAG

Plasmid

#202493

Purpose

Express "M" HaloTag

Depositor

Neha Kamat

Publication

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HaloTag

Tags

1x FLAG and Myristoylation site

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-UAG site)

Plasmid

#170138

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Publication

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(UAG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-702pUC19-tNGFR-P2A-LRBA

Plasmid

#186096

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

LRBA (LRBA Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-469 JUNB-tNGFR

Plasmid

#186060

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

JUNB (JUNB Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Gag-FKBP-Myc

Plasmid

#177909

Purpose

HIV Gag-FKBP-Myc tag

Depositor

Masaharu Somiya

Publication

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HIV Gag (gag )

Tags

FKBP12 and Myc tag

Expression

Mammalian

Promoter

CAG promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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CALID

Plasmid

#169708

Purpose

Cre-activated light-inducible Dre recombinase

Depositor

Dali Li

Publication

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Plasmid

#159800

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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SpyCas9-AIDx

Plasmid

#135343

Purpose

Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Publication

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nSpyCas9-hAIDx

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtPHYB-S_X

Plasmid

#131183

Purpose

Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AtPHYB-S

Expression

Mammalian

Promoter

CMV

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_VfAU1-LOV

Plasmid

#131185

Purpose

Expresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VfAU1-LOV

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_ScPH1-S_X

Plasmid

#131184

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ScPH1-S

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Publication

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RsLP-LOV

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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