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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33,916 results for: eng

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Showing: 6661 - 6680 of 33916 results

  1. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  2. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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  3. pcDNA3-Lyso-PTEN(A4)-mCherry

    Plasmid
    #184054
    Purpose
    Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.
    Depositor
    Jin Zhang
    Article
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Insert
    Lyso-PTEN(A4)-mCherry (PTEN Human)
    Tags
    Lysosome-associated membrane protein 1 (LAMP1). a…
    Expression
    Mammalian
    Mutation
    Ser 380/385 and Thr 382/383 in PTEN mutated to Al…
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184054-plasmid-map-sequence-id-363239

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  6. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid
    #176334
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617.2 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19R, G142D, del156-157, R158G, L452R, T478K, D61…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176334-plasmid-map-sequence-id-347146

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  9. CCR5-SZ190b-sfGFP

    Plasmid
    #162447
    Purpose
    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration
    Depositor
    Shuguang Zhang
    Article
    Qing et al iScience. 2020 Oct 28;23(12):101670. doi
    Insert
    C-C chemokine receptor type 5 (CCR5 Human)
    Expression
    Mammalian
    Mutation
    truncation from aa88 to aa249
    Promoter
    CMV
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162447-plasmid-map-sequence-id-331353

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  11. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid
    #176325
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.7 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176325-plasmid-map-sequence-id-347062

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  13. pVRC8400-SARS-CoV-2-S2P-B.1.351-AVI

    Plasmid
    #176326
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.351 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, 242-244del, R246I, K417N, E484…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176326-plasmid-map-sequence-id-347063

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Showing: 6661 - 6680 of 33916 results