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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 6681 - 6700 of 9221 results

  2. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50593-plasmid-map-sequence-id-451910

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  3. Halo-53BP1 HRD

    Plasmid
    #207087
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207087-plasmid-map-sequence-id-431238

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  6. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

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  8. pMS82

    Plasmid
    #68110
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    Hyg
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Mutation
    Fragment
    Available Since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68110-plasmid-map-sequence-id-248992

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  9. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al bioRxiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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  10. pJAT11

    Plasmid
    #204290
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204290-plasmid-map-sequence-id-405507

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  14. SAGA005

    Plasmid
    #240754
    Purpose
    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15A J23100 BCD_medium - gfp - tetAp15A- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240754-plasmid-map-sequence-id-483915

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Showing: 6681 - 6700 of 9221 results