We narrowed to 10,685 results for: plasmids 101

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pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

Plasmid

#203847

Purpose

Flp-dependent expression of Chronos fused to EGFP

Depositor

Ivo Spiegel

Article

Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

Has Service

AAV2

Insert

Chronos

Use

AAV

Tags

EGFP

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-V5-Lin28A

Plasmid

#51387

Depositor

Narry Kim

Article

Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

Insert

Lin28A (LIN28A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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PpyCAG-NANOG-IB

Plasmid

#60440

Purpose

Transient human NANOG expression

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

NANOG (NANOG Human)

Expression

Mammalian

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OK+9MS-Cas9

Plasmid

#120354

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OK+9MS cassette

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

Plasmid

#126512

Purpose

Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

somArchon1-EGFP-P2A-somCheRiff

Use

AAV

Expression

Mammalian

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_889

Plasmid

#216080

Purpose

ITRs from AAV2, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

ITRs from AAV2

Use

AAV; Destination

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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anti-Notch2_B9-pBIOCAM5

Plasmid

#39348

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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RNF130 shRNA-TRE

Plasmid

#225336

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 shRNA (Rnf130 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-human NMyc-humanER-IRES-mCherry

Plasmid

#73571

Purpose

Mammalian retroviral expression of NMyc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

NMyc (MYCN Human)

Use

Retroviral

Tags

Human ER and IRES-mCherry

Expression

Mammalian

Promoter

MSCV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
MSP1594_2x_NLS

Plasmid

#110625

Purpose

Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
None

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAGH10 - RlucFluc minus 1 HIV-1

Plasmid

#198224

Purpose

Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

Depositor

Isha Jain

Article

Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

Inserts

Renilla luciferase
Firefly luciferase

Expression

Mammalian

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

Plasmid

#187358

Purpose

Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

HPC-4 antibody heavy chain, and 6xHIS tag

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-hCMV1-hNANOG-IV

Plasmid

#60432

Purpose

tet-inducible human NANOG

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

NANOG (NANOG Human)

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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