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pDEST-CMV-C-EGFP

Plasmid

#122844

Purpose

C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Article

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ihSyn1-DIO-tTA (AAV1)

Viral Prep

#99121-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-ihSyn1-DIO-tTA (#99121). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-DIO-tTA plasmid DNA. Cre-dependent expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Promoter

ihSyn1

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only
pAC-CANTHipi

Plasmid

#53301

Purpose

Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtO

Use

Low copy number bacterial cloning vector

Tags

6His

Promoter

Trc

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSL1119 (pDonor)

Plasmid

#160732

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA (Mini-Tn)

Expression

Bacterial

Promoter

N/A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTW334e

Plasmid

#237315

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pN251

Plasmid

#188471

Purpose

Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

enCas12a-HF (gift from Dr. Keith Joung)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DEST (w117-1)

Plasmid

#17454

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(Hygro) TagBFP-hNucleolin

Plasmid

#182592

Purpose

expresses bfp-hNucleolin in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hNucleolin (NCL Human)

Tags

BFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3

Plasmid

#44727

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBAM1

Plasmid

#60487

Purpose

This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDX3X

Plasmid

#116730

Purpose

Lentiviral expression of DDX3X

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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HDAC8 Flag

Plasmid

#13825

Depositor

Eric Verdin

Article

Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

Insert

HDAC8 (HDAC8 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-uTEV3

Plasmid

#234320

Purpose

Mammalian expression of uTEV3 tagged with an eGFP marker

Depositor

Mateo Sánchez

Article

Silva-Rojas et al Nat Biomed Eng. 2025 Jun 5. doi: 10.1038

Insert

eGFP-uTEV3

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Mutations respective to the wild type TEV: S219V …

Promoter

CMV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-GFP

Plasmid

#110834

Purpose

positive control for GFP expression

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

elongation factor 1alpha binding sequence (EFS) at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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SGEP

Plasmid

#111170

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5c-Myc

Plasmid

#186616

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5c (KIF5C Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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