We narrowed to 4,243 results for: Pet2

1. pET28_Caspase-11_humanized-mutant

   Plasmid

   #214315

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   N152K_KLS288YKT_KASIHS348TPRAKA

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_Caspase-11_rat_catalytic domain

   Plasmid

   #214329

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Rat)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_human-pro-IL-18_LES33AAA

   Plasmid

   #214326

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   LES33AAA

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_human-pro-IL-18_DDEN29ENGD

   Plasmid

   #214325

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   DDEN29ENGD

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_human-pro-IL-18_V47N_I48N

   Plasmid

   #214324

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   V47N_I48N

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_Caspase-4_lemur_catalytic domain

   Plasmid

   #214328

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_Caspase-4_rabbit_catalytic domain

   Plasmid

   #214330

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28b-TEV-AcrIC9

   Plasmid

   #207409

   Purpose

   Protein purification vector to produce AcrIC9 in E. coli

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   T7-TEV-AcrIC9
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET23a-H6-TEV-AncSZ

   Plasmid

   #214235

   Purpose

   Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

   Depositor

   Neel Shah

   Article

   Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

   Insert

   Syk kinase (SYK Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence gained through ancestral reconstruction …

   Promoter

   T7

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-DONSON-DEWS

    Plasmid

    #208799

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A; S437A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-DONSON-Δ24

    Plasmid

    #208801

    Purpose

    Xenopus laevis His6-Myc-DONSON (1-24aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Δ24, deletion of 1-24aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-DONSON-DEW

    Plasmid

    #208802

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-DONSON-S437A

    Plasmid

    #208803

    Purpose

    Xenopus laevis His6-Myc-DONSON (S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    S437A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-DONSON-ΔD1

    Plasmid

    #208804

    Purpose

    Xenopus laevis His6-DONSON (1-155aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    ΔD1, deletion of 1-155aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-6xHis-TrxF

    Plasmid

    #207454

    Purpose

    E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

    Depositor

    Joseph Jez, Sarah Stainbrook

    Article

    Stainbrook et al bioRxiv 2022.08.24.505184

    Insert

    Thioredoxin F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    T7

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-Flag-RBM24-1~93

    Plasmid

    #208633

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 1~93, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-eIF4E-TwStrep

    Plasmid

    #208638

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

    Depositor

    Quanfu Ma

    Insert

    human eIF4E (ENST00000450253) (EIF4E Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-RPL3-Twstrep

    Plasmid

    #208623

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) (RPL3 Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24a-NUbo-E3(63)-His6

    Plasmid

    #212717

    Purpose

    Bacterial expression of NUbo-E3(63)-His6

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Tags

    6xHis and NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-NUbo-E3(48)-His6

    Plasmid

    #212714

    Purpose

    Bacterial expression of NUbo-E3(48)-His6

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Tags

    6xHis and NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-hXRCC1pd-HIS

    Plasmid

    #206036

    Purpose

    Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    T7

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pet28b-His_RAD23A-UBA2

    Plasmid

    #201550

    Purpose

    Expresses UBA2 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 311-363 of DNA polymerase i…

    Promoter

    T7

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28 mePOU-POU

    Plasmid

    #206392

    Purpose

    Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    ePOU
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28 mOct4-POU

    Plasmid

    #206393

    Purpose

    Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    POU DNA binding domain only

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a LZ control-FLAG

    Plasmid

    #199678

    Purpose

    Bacterial expression of Leucine Zipper (LZ) control-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper control
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a LZ LED WT-FLAG

    Plasmid

    #199679

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED WT (GPR155 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET23PI-Sce ESARC

    Plasmid

    #195607

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

    Promoter

    T7

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28 em-blue-shift.2

    Plasmid

    #191901

    Purpose

    Encodes a GFP design, em-blue-shift.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    em-blue-shift.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72A T108I Y145F V150I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a Cdk2ap1CAN-MutTER

    Plasmid

    #178033

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Changed T(108)A, E(109)A, R(110)A

    Promoter

    T7LacI

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28-MBP-CI2F

    Plasmid

    #191254

    Purpose

    Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

    Depositor

    Arthur Laganowsky

    Article

    Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

    Insert

    Chymotrypsin Inhibitor 2
    

    Use

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET24a-LaM4-2xTS

    Plasmid

    #162779

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-matNODALvar-HIS

    Plasmid

    #115265

    Purpose

    For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL variant mature peptide (NODAL Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21a Sa-iPGM-bio

    Plasmid

    #179523

    Purpose

    Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    S. aureus iPGM bio-6His
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21a Mo-iPGM-bio

    Plasmid

    #179614

    Purpose

    Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    M. orale iPGM bio-6His
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21a Hp-iPGM-bio

    Plasmid

    #179615

    Purpose

    Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    H. pylori iPGM bio-6His
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-eGFP-Pep-H

    Plasmid

    #185788

    Purpose

    Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    eGFP-Pep4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28b-MBP-bhaB5

    Plasmid

    #167812

    Purpose

    Expresses His6- and MBP-tagged BhaB5 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaB5
    

    Use

    Tags

    His6, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21a(+)_co_L-HicDH

    Plasmid

    #185453

    Purpose

    CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

    Depositor

    Wolfgang Kroutil

    Article

    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

    Insert

    L-HicDH from Lactobacillus confusus DSM 20196
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

    Plasmid

    #183387

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    redesigned Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    ANR261ENP, D315H, M318K, T352K

    Promoter

    T7

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge