We narrowed to 7,293 results for: Ank

1. pAAV_COL4A5_self-cleavingCas9

   Plasmid

   #130282

   Purpose

   Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   spCas9
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. vPyACR_2164382

   Plasmid

   #153029

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_2164382
   

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_2164382 was C-terminally truncated to incl…

   Promoter

   CMV (+ enhancer)

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. vPyACR_21821

   Plasmid

   #153030

   Purpose

   Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

   Insert

   vPyACR_21821
   

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   vPyACR_21821 was C-terminally truncated to includ…

   Promoter

   CMV (+ enhancer)

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB139 - pL0_ZCT1 (CDS1)

   Plasmid

   #123184

   Purpose

   Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   ZCT1 from Catharanthus roseus
   

   Use

   Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB142 - pL0_ORCA3 (CDS1)

   Plasmid

   #123185

   Purpose

   Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   ORCA3 from Catharanthus roseus
   

   Use

   Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDX2 ObLiGaRe Donor vector/EPB64

   Plasmid

   #90017

   Purpose

   Donor vector for ObLiGaRe/ZFN mediated targeting to CDX2 exon1 locus

   Depositor

   Eric-Paul Bennett

   Article

   Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

   Insert

   MCS flanked by inverted CDX2 ZFN binding sites (CDX2 Human)
   

   Expression

   Bacterial

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. STK32C gRNA (BRDN0001146198)

   Plasmid

   #76931

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK32C

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK32C (STK32C Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. STK32C gRNA (BRDN0001146377)

   Plasmid

   #76932

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK32C

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK32C (STK32C Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. STK32C gRNA (BRDN0001149411)

   Plasmid

   #76933

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK32C

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK32C (STK32C Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIX_sh_hMIB2_#9

    Plasmid

    #75255

    Purpose

    retroviral shRNA vector against human MIB2, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against MIB2 (human) (MIB2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. mGDF8 donor CFP-hDF

    Plasmid

    #72590

    Purpose

    Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. hGDF8 donor CFP-hDF

    Plasmid

    #72591

    Purpose

    Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET01-TAF5-ex8-Minigene-SRSF1 motif WT

    Plasmid

    #218972

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with wild type SRSF1 motif (TCAGAGGA)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with wild type SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Expression

    Mammalian

    Promoter

    RSV LTR

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. C-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58694

    Purpose

    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Csplit Intein and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscAAV-Sox2-mStr

    Plasmid

    #135617

    Purpose

    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-mStrawberry

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Sox2-CreERT2

    Plasmid

    #135618

    Purpose

    Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-CreERT2

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCG_IFITM3

    Plasmid

    #179957

    Purpose

    Expression vector for human IFITM3.

    Depositor

    Frank Kirchhoff, Konstantin Sparrer

    Article

    Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

    Insert

    IFITM3 (IFITM3 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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