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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Article

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMaRSC

Plasmid

#89189

Purpose

The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

Flag-L274G-T2A-mCherry (Mars1 Mouse)

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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Src-Y530F

Plasmid

#124659

Purpose

Human c-Src with Y530F mutation

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

cSrc (SRC Human)

Expression

Mammalian

Mutation

Y530F

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-IL6

Plasmid

#111933

Purpose

Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Interleukin 6 (IL6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-MCC

Plasmid

#133307

Purpose

"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

microcin-V bacteriocin cassette (cvaC E. coli)

Use

Synthetic Biology

Expression

Bacterial

Mutation

N112D (please see depositors comments)

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMax_ScaI_BFP

Plasmid

#177824

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP

Expression

Mammalian

Mutation

5' inserted ScaI RE cut site

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-EEA1

Plasmid

#109560

Purpose

Multisite gateway vector for 5' tagging with early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

early endosome associated 1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-RAB11A

Plasmid

#176489

Purpose

For lentiviral transduction of 3FLAG-RAB11A

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

RAB11A (RAB11A Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA63

Plasmid

#25641

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 63 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pAM1

Plasmid

#51401

Purpose

Expresses truncated version of NLL-MetRS from endogenous MetG promoter

Depositor

David Tirrell

Article

Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Insert

MetRS (metG E coli)

Mutation

L13N, Y260L, H301L; Please note that a truncated …

Promoter

MetG

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 2TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-mCherry-CLCN3

Plasmid

#225514

Purpose

Lentiviral mCherry-CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Use

Lentiviral

Tags

mCherry

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCD5-PR8-TEV-sfGFP-twinStrep

Plasmid

#157933

Purpose

Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

Insert

A/PuertoRico/8/34 hemagglutinin (HA Synthetic)

Tags

sfGFP & TwinStrep

Expression

Mammalian

Mutation

E190D

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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spCas9-NLS-MAV

Plasmid

#124212

Purpose

SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

Depositor

Uri David Akavia

Article

Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

Insert

cas9

Use

CRISPR

Tags

His6, MBP, TEV Cleavage site, and two copies of S…

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-Rab7

Plasmid

#64210

Purpose

Rab7 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab7 (Rab7 Mouse)

Tags

FRB and triple myc tag (3myc)

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DNM2-R465W

Plasmid

#155262

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Tags

HA

Expression

Mammalian

Mutation

Arginine 465 to Tryptophan

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA -CLCN3-3xFlag

Plasmid

#225506

Purpose

CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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