We narrowed to 10,815 results for: Ski

1. pJL1-mRFP1 gRNA

   Plasmid

   #117052

   Purpose

   In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   anti-mRFP1 SpCas9 gRNA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

   Plasmid

   #130709

   Purpose

   Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
   

   Tags

   NTOM20 and SNAP-Tag

   Expression

   Mammalian

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-R-iLACCO1.1

   Plasmid

   #208020

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1.1
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ10714

   Plasmid

   #183959

   Purpose

   U6-crKlf4-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Klf4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

   Plasmid

   #194688

   Purpose

   hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-sgRNA_Ago1_1

   Plasmid

   #73533

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-sgRNA_Ago1_2

   Plasmid

   #73534

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pCIBN-EYFP

   Plasmid

   #103815

   Purpose

   Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-pr-mEos4b (mEosFP4b-V69T)

   Plasmid

   #99226

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos4b
   

   Expression

   Bacterial

   Mutation

   V69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-gp120(BaL)-CD4

    Plasmid

    #98957

    Purpose

    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    HIV-1 (BaL) gp120
    

    Tags

    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TXD13-CD4d3+4-bio

    Plasmid

    #73108

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding TXD13 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    TXD13 (TMX4 Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. bio-CD4d3+4-ADL1

    Plasmid

    #73090

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ADL1 with bio-rat CD4d3+4

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ADL1 (NCEH1 Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. LENTICRISPR-PGAM1_sgRNA2

    Plasmid

    #201615

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1 FRB-myc-RagA

    Plasmid

    #112740

    Purpose

    express FRB-myc-RagA

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagA (RRAGA Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-iCre-2A-venus

    Plasmid

    #182499

    Purpose

    Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    iCre-2A-Venus
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    fragment of histidine decarboxylase gene promoter…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Supt6h-SH2

    Plasmid

    #46518

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Supt6h (SUPT6H Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1317-1441

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-TPD52

    Plasmid

    #172452

    Purpose

    Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIBN-mCherry

    Plasmid

    #103816

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Arp2_pLib

    Plasmid

    #173676

    Purpose

    Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    Arp2 (ACTR2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    U6

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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