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pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244102

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244089

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244084

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244090

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.HaloTag

Plasmid

#244085

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

Plasmid

#239608

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-R-eLACCO2

Plasmid

#223255

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2.1

Expression

Bacterial

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-R-eLACCO2.1

Plasmid

#223256

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

deLACCO1

Expression

Bacterial

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-R-deLACCOctrl

Plasmid

#223257

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-iLACCO1

Expression

Bacterial

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-thrombin-TEX264(28-313aa)

Plasmid

#227714

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

TEX264(28-313aa) (TEX264 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-thrombin-FAM134C(250-466aa)

Plasmid

#227715

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FAM134C(250-466aa) (RETREG3 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/A25T

Plasmid

#238115

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S/S85C/A25T

Promoter

T7

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR A25T/I26V)

Plasmid

#238117

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

A25T/I26V

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M7138

Plasmid

#219774

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of ORCA3 promoter and terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M7691

Plasmid

#219775

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of WRKY70 promoter and terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pNK3558

Plasmid

#219776

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of p35S promoter and act2 terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | p35S-nnLuz-act2

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | p35S-nnLuz-act2

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-FLAG-coK8

Plasmid

#235542

Purpose

To express tagBFP-2A-FLAG-coK8 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

K8

Use

Lentiviral

Tags

FLAG

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_IFNB1_gRNA_1

Plasmid

#235532

Purpose

gRNA against human IFNB1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IFNB1 (IFNB1 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_IFNB1_gRNA_3

Plasmid

#235533

Purpose

gRNA against human IFNB1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IFNB1 (IFNB1 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-coICP0-∆USP7

Plasmid

#235540

Purpose

To express tagBFP-2A-coICP0-∆USP7 (ICP0 ∆618-633, co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0-∆USP7

Use

Lentiviral

Mutation

Deletion of region ∆618-633.

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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