We narrowed to 92,501 results for: lias

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pLIX402 caPHF2

Plasmid

#91701

Purpose

inducible lentiviral expression of constitutively active PHF2

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

caPHF2; S757D, S899D, S954D, S1056D

Promoter

TRE promoter, Tet ON

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

Plasmid

#62131

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV LacZ L5-L2

Plasmid

#62166

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

LacZ

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPKm-196

Plasmid

#90511

Purpose

pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PIF6-DBD

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf196

Plasmid

#12884

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf196

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

Plasmid

#84260

Purpose

Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgSV40
PYL1-KRAB

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hRREB1-RFP

Plasmid

#22924

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-0.66kb-EGFP

Plasmid

#153165

Purpose

Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

Plasmid

#108848

Purpose

Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

pMag,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPP300-T2A-GFP-shP53

Plasmid

#102896

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPP300-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 mCherry L3-L2

Plasmid

#62150

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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Flag Snail 8SA

Plasmid

#16222

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pAd FLAG HDAC5 S259A/S498A

Plasmid

#32208

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

S259A/S498A

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf117

Plasmid

#12805

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf117

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5

Plasmid

#161555

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMXSIP-GPR35-FLAG-APEX

Plasmid

#204706

Purpose

expresses GPR35-FLAG-APEX in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

G protein-coupled receptor 35 (GPR35 Human)

Tags

FLAG, APEX

Expression

Mammalian

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit

Plasmid

#113443

Purpose

ProteinA-mCitrine control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Actin.CIB.GFP

Plasmid

#133618

Purpose

Expresses beta actin - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

Plasmid

#108846

Purpose

Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

Plasmid

#62128

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-dCpf1-BE0

Plasmid

#107678

Purpose

Expresses dLbCpf1-BE0 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0

Expression

Mammalian

Mutation

D832A/E925/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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-111 hTF m2

Plasmid

#15449

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 sites mutated

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only
GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

Plasmid

#108845

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

Plasmid

#108843

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-455

Plasmid

#26319

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-455 (MIR455 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only