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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Tags

mEos2

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAS132

Plasmid

#211503

Purpose

AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mCherry

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-SQSTM1

Plasmid

#223715

Purpose

Expresses Flag-tagged SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSPObooster

Plasmid

#216160

Purpose

Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

Depositor

Grant Brown

Article

Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

Inserts

MKT1(30G) (MKT1 Budding Yeast)
RME1(ins-308A) (RME1 Budding Yeast)

Expression

Yeast

Mutation

MKT1 gene containing an aspartic acid to glycine …

Promoter

endogenous promoter

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28

Plasmid

#45569

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-ANXA6-mScarlet

Plasmid

#215475

Purpose

Expression vector with Annexin A6 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ANXA6 (ANXA6 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1A-CD19.28H.28TM.28.BB.3z

Plasmid

#200680

Purpose

Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-TurboID-3xFLAG

Plasmid

#186814

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

TurboID-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Vinculin

Plasmid

#50513

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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HA-EglN1-pcDNA3

Plasmid

#18963

Depositor

William Kaelin

Article

Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

Egg laying Nine - 1 (EGLN1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_mCH

Plasmid

#127109

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

mCherry

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_S5E2_Chrimson_tdTomato

Plasmid

#219432

Purpose

AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

Depositor

Gordon Fishell

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chrimson-tdTomato

Use

AAV

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP35_EBOV

Plasmid

#103050

Purpose

Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

Plasmid

#69901

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B-Rigor

Plasmid

#172204

Purpose

Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pOSIP-KO (KanR, 186)

Plasmid

#45985

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

Plasmid

#52092

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-PLIN3

Plasmid

#161918

Purpose

Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Perilipin-3 (PLIN3 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mNG

Plasmid

#179819

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti PGK V5-LUC Neo (w623-2)

Plasmid

#21471

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

V5-LUC

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (1R)

Plasmid

#29664

Depositor

Scott Gradia

Type

Empty backbone

Tags

Strep II and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-SpG-P2A-hMLH1dn

Plasmid

#211550

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG-P2A-hMLH1dn

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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