165,198 results

1. CMV-OMM-jRCaMP1b

   Plasmid

   #127871

   Purpose

   Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Use

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pComb3HTT

   Plasmid

   #63900

   Purpose

   Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP-SFFV-mtagBFP-2A STING

   Plasmid

   #102586

   Purpose

   Lentivector to express STING and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGIPZ-PD-L1-EGFP

   Plasmid

   #120933

   Purpose

   Express PD-L1-EGFP fusion protein in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

   Insert

   PD-L1-EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV500 A-FOS

   Plasmid

   #33353

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   FOS (Fos Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative of c-Jun; heterodimerizes and i…

   Promoter

   CMV

   Available since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

   Plasmid

   #65417

   Purpose

   AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Has Service

   AAV8

   Insert

   hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
   

   Use

   AAV

   Tags

   HA and mCitrine

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE-zCas9

   Plasmid

   #101739

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9-UGI-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. co luxC pcDNA3.1(+)

    Plasmid

    #129087

    Purpose

    Expression of luxC in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. co luxD pcDNA3.1(+)

    Plasmid

    #129088

    Purpose

    Expression of luxD in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. co frp pcDNA3.1(+)

    Plasmid

    #129090

    Purpose

    Expression of frp in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co frp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSicoR-eIF1a-GFP-RPL10a

    Plasmid

    #159890

    Purpose

    Expresses GFP-RPL10a for ribosomal immunoprecipitation

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

    Insert

    RPL10a (RPL10A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    eIF1a

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJH-MBP-CbAgo

    Plasmid

    #127707

    Purpose

    pET His6 MBP TEV CbAgo

    Depositor

    John van der Oost

    Article

    Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

    Insert

    CbAgo
    

    Use

    Tags

    MPB

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-Kvbeta2/KCNAB2 K+ channel [K17/70R] (Recombinant Antibody)

    Antibody

    #180102-rAb

    Purpose

    Anti-Kvbeta2/KCNAB2 K+ channel (Mouse) recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Recommended Applications

    Immunohistochemistry and Western Blot

    Reactivity

    Human, Mouse, Rat, and Zebrafish

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available since

    March 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

17. pLenti-CMV-Puro-PNPT1 S484A

    Plasmid

    #223316

    Purpose

    Lentiviral vector expressing human PNPT1 S484A variant

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed serine 484 to alanine

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR_Gal4UAS_Blinatumomab_PGK_mCherry

    Plasmid

    #85437

    Purpose

    [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Blinatumomab
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-link-ymScarletI3-Kan

    Plasmid

    #224077

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    ymScarletI3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SmR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBAD_mCherry_GFP_-+

    Plasmid

    #187390

    Purpose

    arabinose-inducible expression of GFP and mCherry

    Depositor

    Roi Avraham

    Insert

    GFP, mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF1230

    Plasmid

    #144092

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    AFF3 (AFF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-HA-hIFITM1

    Plasmid

    #58399

    Purpose

    Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM1 (IFITM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-Ets2

    Plasmid

    #28128

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    Ets2 (ETS2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-3xFLAG-V5-ccdB

    Plasmid

    #87064

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. co luxA pcDNA3.1(+)

    Plasmid

    #129085

    Purpose

    Expression of luxA in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. co luxB pcDNA3.1(+)

    Plasmid

    #129086

    Purpose

    Expression of luxB in mammalian cells

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

    Insert

    co luxB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dyn2-pmCherryN1

    Plasmid

    #27689

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Dynamin 2 (DNM2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRK-ATF4

    Plasmid

    #26114

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mEos3.2GG

    Plasmid

    #203756

    Purpose

    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    KRas c-term (KRAS Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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