We narrowed to 20,932 results for: REN

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

Plasmid

#189755

Purpose

Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-ERSE1-luc2P-Hygro

Plasmid

#101789

Purpose

Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xERSE-I

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Tags

T2A peptide

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6s.WPRE.SV40

Plasmid

#100844

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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eforRed chromoprotein

Plasmid

#117837

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, eforRed

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_OT1.0

Plasmid

#185386

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

hSyn

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2

Plasmid

#224769

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pCXN2-BCL6 (CXN-BCL6)

Plasmid

#40346

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

BCL6 (BCL6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

pCAG

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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spisPink chromoprotein

Plasmid

#117839

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, spisPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSIE1

Plasmid

#136355

Purpose

Selection by Inducible Effectors

Depositor

Andrew Goodman

Article

Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

Inserts

Type VI effector bfe1
Type VI effector bte1
BT_4676
tetR

Use

Unspecified

Mutation

ATG removed to add signal sequence before gene

Promoter

P1TDP-GHO23

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR2

Plasmid

#117929

Purpose

ADAR2 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR2 (ADARB1 Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hGfap-R-CaMP1.07-WPRE-SV40

Plasmid

#164140

Purpose

AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

Depositor

Hirokazu Hirai, Hajime Hirase

Article

Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

Insert

R-CaMP1.07

Use

AAV

Promoter

hGFAP

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R273H

Plasmid

#22934

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R273H Mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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