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pGAD-Atg17(∆15-106)

Plasmid

#121515

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 15-106

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(∆186-236)

Plasmid

#121519

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 186-236

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(∆266-312)

Plasmid

#121521

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 266-312

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS Exon

Plasmid

#110119

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati Exons 1-3

Plasmid

#110113

Purpose

Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104618

Purpose

Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

hsp70-Syn21-FLAG-mCerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10

Plasmid

#104616

Purpose

Expresses HA tagged Citrine upon binding of TALE1 to VAS1

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

Syn21-Citrine-HA-P10-MhcSD

Tags

HA

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRRG312-slc1a-5

Plasmid

#99102

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

slc1a-5 (also known as excitatory amino acid transporter) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG94-runt-1

Plasmid

#99089

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 (also known as runt)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

Runt-1 (also known as runt) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG217-soxB

Plasmid

#99086

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also known as soxB1-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

soxB (also known as soxB1-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG325-Unc-9

Plasmid

#99084

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Unc-9 (also known as h.26.12f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Unc-9 (also known as h.26.12f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG246-MCP

Plasmid

#99083

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea MCP (also known as h.45.8g)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

MCP (also known as h.45.8g) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG208-ActR1

Plasmid

#99077

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ActR1 (activin receptor 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ActR1 (activin receptor 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG128-activin

Plasmid

#99075

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Activin (also known as activin 2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Activin (also known as activin 2) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1::CDKA;1:YFP

Plasmid

#80685

Purpose

binary Expression vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

Insert

CDKA;1 (CDC2 Mustard Weed)

Tags

YFP

Expression

Plant

Promoter

ProCDKA;1

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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GST-peripherin-CTS

Plasmid

#86070

Purpose

A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Peripherin (prph2.S Frog)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 317-336 of peripherin

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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GST-prRDH-CTS

Plasmid

#86069

Purpose

A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Retinol dehydrogenase (RDH8 Bovine)

Tags

GST

Expression

Bacterial

Mutation

the insert is 297-312 of RDH8 (Bos taurus)

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHJ12: CadC-VHH-Caffeine (No linker)

Plasmid

#108244

Purpose

Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker

Use

Synthetic Biology

Promoter

pLacO1

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Promoter

gfaABC1D (astrocyte specific)

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHJ4: LexA-VHH-Caffeine

Plasmid

#108242

Purpose

LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with VHH-Caffeine antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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